The K+ Channel KZM1 Mediates Potassium Uptake into the Phloem and Guard Cells of the C4 Grass Zea mays

In search of K+ channel genes expressed in the leaf of the C4 plant Zea mays, we isolated the cDNA of KZM1 (forK+ channel Zeamays 1). KZM1 showed highest similarity to the Arabidopsis K+ channels KAT1 and KAT2, which are localized in guard cells and phloem. When expressed in Xenopus oocytes, KZM1 ex...

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Published in:The Journal of biological chemistry 2003-05, Vol.278 (19), p.16973-16981
Main Authors: Philippar, Katrin, Büchsenschütz, Kai, Abshagen, Maike, Fuchs, Ines, Geiger, Dietmar, Lacombe, Benoit, Hedrich, Rainer
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Ion Transport
Molecular Sequence Data
Patch-Clamp Techniques
Periodicity
Potassium Channels - physiology
Xenopus
Zea mays - physiology
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Summary:In search of K+ channel genes expressed in the leaf of the C4 plant Zea mays, we isolated the cDNA of KZM1 (forK+ channel Zeamays 1). KZM1 showed highest similarity to the Arabidopsis K+ channels KAT1 and KAT2, which are localized in guard cells and phloem. When expressed in Xenopus oocytes, KZM1 exhibited the characteristic features of an inward-rectifying, potassium-selective channel. In contrast to KAT1- and KAT2-type K+ channels, however, KZM1 currents were insensitive to external pH changes. Northern blot analyses identified the leaf, nodes, and silks as sites ofKZM1 expression. Following the separation of maize leaves into epidermal, mesophyll, and vascular fractions, quantitative real-time reverse transcriptase-PCR allowed us to localizeKZM1 transcripts predominantly in vascular strands and the epidermis. Cell tissue separation and KZM1 localization were followed with marker genes such as the bundle sheath-specific ribulose-1,5-bisphosphate carboxylase, the phloem K+channel ZMK2, and the putative sucrose transporterZmSUT1. When expressed in Xenopus oocytes, ZmSUT1 mediated proton-coupled sucrose symport. Coexpression of ZmSUT1 with the phloem K+ channels KZM1 and ZMK2 revealed that ZMK2 is able to stabilize the membrane potential during phloem loading/unloading processes and KZM1 to mediate K+ uptake. During leaf development, sink-source transitions, and diurnal changes,KZM1 is constitutively expressed, pointing to a housekeeping function of this channel in K+ homeostasis of the maize leaf. Therefore, the voltage-dependent K+-uptake channel KZM1 seems to mediate K+retrieval and K+ loading into the phloem as well as K+-dependent stomatal opening.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M212720200