Nectomys squamipes Microsatellites and Homologous Loci in Sigmodontine Rodents

Three monomorphic and four highly polymorphic microsatellites of Nectomys squamipes were isolated and characterised in a sample of 141 specimens from eight different Brazilian localities. These seven microsatellites and four others previously described in this species were tested in seven other nonf...

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Bibliographic Details
Published in:The Journal of heredity 2003-03, Vol.94 (2), p.171-174
Main Authors: Maroja, L. S., Almeida, F. C., Cerqueira, R., Seuánez, H. N., Moreira, M. A. M.
Format: Article
Language:English
Subjects:
Animals
Genetic Markers
Heredity
Microsatellite Repeats
Rats
Rodentia - genetics
Rodents
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Summary:Three monomorphic and four highly polymorphic microsatellites of Nectomys squamipes were isolated and characterised in a sample of 141 specimens from eight different Brazilian localities. These seven microsatellites and four others previously described in this species were tested in seven other nonfocus sigmodontine species. At least three loci were successfully amplified in every species, but none was amplified in all species. All sequenced products in nonfocus species showed (GT)n motifs as in N. squamipes. Several loci were amplified in Nectomys rattus and Oligoryzomys nigripes, while absence of PCR products was observed more frequently in Oxymycterus dasythricus and Akodon cursor. Two of three monomorphic loci in N. squamipes were polymorphic in other species.
ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/esg017