Failure of a recombinant Schistosoma bovis-derived glutathione S-transferase to protect cattle against experimental Fasciola hepatica infection

The potential of a recombinant Schistosoma bovis 28-kDa glutathione S-transferase (rSb28GST) to protect cattle against Fasciola hepatica was tested in a vaccination trial. Thirty two calves were randomly divided into four groups of eight animals. Calves of the three vaccine groups received two intra...

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Bibliographic Details
Published in:Veterinary parasitology 2003-04, Vol.113 (2), p.135-144
Main Authors: De Bont, J, Claerebout, E, Riveau, G, Schacht, A.M, Smets, K, Conder, G, Brake, D.A, Capron, A, Vercruysse, J
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Animals
Antibodies, Helminth - blood
Cattle
Cattle Diseases - immunology
Cattle Diseases - parasitology
Cattle Diseases - prevention & control
Cattle-trematoda
Eosinophilia - immunology
Fasciola hepatica
Fasciola hepatica - growth & development
Fasciola hepatica - immunology
Fascioliasis - immunology
Fascioliasis - parasitology
Fascioliasis - prevention & control
Fascioliasis - veterinary
Feces - parasitology
Female
gamma-Glutamyltransferase - blood
Glutathione S-transferase
Glutathione Transferase - immunology
Glutathione Transferase - pharmacology
Immunization - veterinary
Parasite Egg Count - veterinary
Random Allocation
Recombinant Proteins - immunology
Recombinant Proteins - pharmacology
Schistosoma - enzymology
Schistosoma - immunology
Vaccine
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Summary:The potential of a recombinant Schistosoma bovis 28-kDa glutathione S-transferase (rSb28GST) to protect cattle against Fasciola hepatica was tested in a vaccination trial. Thirty two calves were randomly divided into four groups of eight animals. Calves of the three vaccine groups received two intramuscular injections at 3 weeks interval, of 0.250 mg rSb28GST in either aluminium hydroxide (Al(OH) 3), Quil A, or PBS emulsified in an equal volume of Freund’s complete adjuvant (FCA). Animals of the control group received injections of Al(OH) 3/PBS only. All animals were challenged orally with a total of 360 metacercariae of F. hepatica, spread over 6 weeks. All groups of vaccinated animals produced measurable IgG antibody titers to rSb28GST after vaccination. Animals immunised with FCA adjuvanted vaccine had the highest and more durable antibody titers and only sera from this group recognised an approximately 24 kDa protein band from F. hepatica, that is thought to be a F. hepatica GST. Despite a good antibody response differences in cumulative faecal egg output between the groups were not statistically significant. In addition, no significant difference was found between groups in terms of total worm numbers or percentage of immature flukes recovered at necropsy. In conclusion, the recombinant S. bovis 28 kDa GST was not found to adequately protect cattle against experimental F. hepatica challenge, using either aluminium hydroxide, Quil A or FCA as adjuvant.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(02)00450-8