Differential nitric oxide production by chicken immune cells

Nitric oxide is a rapidly reacting free radical which has cytotoxic effects during inflammatory responses and regulatory effects as a component of signal transduction cascades. We quantified the production of nitrite, a stable metabolite of nitric oxide, in chicken heterophils, monocytes and macroph...

Full description

Saved in:
Bibliographic Details
Published in:Developmental and comparative immunology 2003-06, Vol.27 (6), p.603-610
Main Authors: Crippen, Tawni L, Sheffield, Cynthia L, He, Haiqi, Lowry, Virginia K, Kogut, Michael H
Format: Article
Language:English
Subjects:
Animals
Chicken
Chickens - metabolism
HD11 cells
Heterophil
Immune System - metabolism
Macrophages - metabolism
Monocytes
Monocytes - metabolism
Nitric oxide
Nitric Oxide - biosynthesis
Nitrites - metabolism
S-methylisothiourea
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nitric oxide is a rapidly reacting free radical which has cytotoxic effects during inflammatory responses and regulatory effects as a component of signal transduction cascades. We quantified the production of nitrite, a stable metabolite of nitric oxide, in chicken heterophils, monocytes and macrophages after stimulation by IFNγ, LPS and killed bacteria. Our results demonstrate a differential production of nitrite over 72 h by chicken peripheral blood heterophils, monocytes and the chicken macrophage cell line (HD11). HD11 cells produced an average of 10 fold more nitrite in comparison to monocytes and 30 fold more than heterophils upon stimulation. This production could be inhibited by S-methylisothiourea indicating that the inducible nitric oxide synthase enzyme was participating in the pathway leading to nitrite production.
ISSN:0145-305X
1879-0089
DOI:10.1016/S0145-305X(03)00035-1