Improved catalytic performance of Bacillus megaterium epoxide hydrolase in a medium containing Tween-80

A new epoxide hydrolase with high enantioselectivity toward (R)-glycidyl phenyl ether (R-GPE) was partially purified from Bacillus megaterium strain ECU1001. The maximum activity of the isolated enzyme was observed at 30 degrees C and pH 6.5 in a buffer system with 5% (v/v) of DMSO as a cosolvent. T...

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Bibliographic Details
Published in:Biotechnology progress 2003-03, Vol.19 (2), p.652-654
Main Authors: GONG, Peng-Fei, XU, Jian-He, TANG, Yan-Fa, WU, Hui-Yuan
Format: Article
Language:English
Subjects:
Bacillus megaterium - chemistry
Bacillus megaterium - enzymology
Biological and medical sciences
Catalysis
Coenzymes - chemistry
Coenzymes - metabolism
Dimethyl Sulfoxide - chemistry
Dimethyl Sulfoxide - pharmacology
Enzyme Activation - drug effects
Enzyme Activators - chemistry
Enzyme Stability - drug effects
Epoxide Hydrolases - biosynthesis
Epoxide Hydrolases - chemistry
Fundamental and applied biological sciences. Psychology
Isomerism
Polysorbates - chemistry
Polysorbates - pharmacology
Solutions
Solvents - chemistry
Substrate Specificity
Temperature
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Summary:A new epoxide hydrolase with high enantioselectivity toward (R)-glycidyl phenyl ether (R-GPE) was partially purified from Bacillus megaterium strain ECU1001. The maximum activity of the isolated enzyme was observed at 30 degrees C and pH 6.5 in a buffer system with 5% (v/v) of DMSO as a cosolvent. The enzyme was quite stable at pH 7.5 and retained full activity after incubation at 40 degrees C for 6 h. Interestingly, when the cosolvent DMSO was replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate, the apparent activity of the epoxide hydrolase significantly increased by about 1.8-fold, while the temperature optimum shifted from 30 to 40 degrees C and the half-life of the enzyme at 50 degrees C increased by 2.5 times. The enzymatic hydrolysis of rac-GPE was highly enantioselective, with an E-value (enantiomeric ratio) of 69.3 in the Tween-80 emulsion system, which is obviously higher than that (41.2) observed in the DMSO-containing system.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp020293v