Xite, X-Inactivation Intergenic Transcription Elements that Regulate the Probability of Choice

Allelic expression differences contribute to phenotypic variation. In X chromosome inactivation (XCI), unfavorable XCI ratios promote X-linked disease penetrance in females. During XCI, one X is randomly silenced by Xist. X chromosome choice is determined by asymmetric expression of Tsix whose antis...

Full description

Saved in:
Bibliographic Details
Published in:Molecular cell 2003-03, Vol.11 (3), p.731-743
Main Authors: Ogawa, Yuya, Lee, Jeannie T.
Format: Article
Language:English
Subjects:
Alleles
Animals
Bacterial Outer Membrane Proteins - genetics
Base Sequence
CpG Islands
Deoxyribonuclease I - metabolism
Down-Regulation
Escherichia coli Proteins
Exons
Female
Gene Deletion
Gene Expression Regulation
Genes, Regulator
In Situ Hybridization, Fluorescence
Male
Mice
Mice, Knockout
Models, Genetic
Molecular Sequence Data
Protein Binding
Receptors, Virus
Reverse Transcriptase Polymerase Chain Reaction
RNA - metabolism
RNA, Long Noncoding
RNA, Messenger - metabolism
RNA, Untranslated - genetics
Transcription Factors - genetics
Transcription, Genetic
X Chromosome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Allelic expression differences contribute to phenotypic variation. In X chromosome inactivation (XCI), unfavorable XCI ratios promote X-linked disease penetrance in females. During XCI, one X is randomly silenced by Xist. X chromosome choice is determined by asymmetric expression of Tsix whose antisense action represses Xist. Here, we discover a cis element in the mouse X-inactivation center that regulates Tsix. Xite harbors intergenic transcription start sites and DNaseI hypersensitive sites with allelic differences. At the onset of XCI, deleting Xite downregulates Tsix in cis and skews XCI ratios, suggesting that Xite promotes Tsix persistence on the active X. Truncating Xite RNA is inconsequential, indicating that Xite action does not require intact transcripts. We propose that allele-specific Xite action promotes Tsix asymmetry and generates X chromosome inequality. Therefore, Xite is a candidate for the Xce, the classical modifier of XCI ratios.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(03)00063-7