Endothelial Modulation of the Ouabain-Induced Contraction in Human Placental Vessels

Ouabain (1×10-3×10 M) elicited concentration-dependent vasoconstriction in human placental arteries and veins. These responses, but not those produced by 10 M 5-hydroxytryptamine, were increased after the removal of vascular endothelium. In placental arteries, the respective blockade of cyclooxygena...

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Bibliographic Details
Published in:Circulation research 1992-10, Vol.71 (4), p.943-950
Main Authors: Sáinchez-Ferrer, Carlos F, Fernández-Alfonso, María S, Ponte, Ana, Casado, Miguel A, Gonzáilez, Rita, Rodríguez-Mañas, Leocadio, Pareja, Ana, Marín, Jesus
Format: Article
Language:English
Subjects:
5,8,11,14-Eicosatetraynoic Acid - pharmacology
Adult
Arginine - analogs & derivatives
Arteries - drug effects
Biological and medical sciences
Biological Assay
Blood Vessels - drug effects
Cardiac & Cardiovascular Systems
Cardiovascular System & Cardiology
Embryology: invertebrates and vertebrates. Teratology
Endothelium, Vascular - physiology
Female
Fetal membranes
Fundamental and applied biological sciences. Psychology
General aspects. Development. Fetal membranes
Hematology
Humans
Indomethacin - pharmacology
Life Sciences & Biomedicine
Lipoxygenase Inhibitors - pharmacology
Muscle Contraction - drug effects
omega-N-Methylarginine
Ouabain - pharmacology
Oxyhemoglobins - analysis
Peripheral Vascular Disease
Placenta - blood supply
Pyrazoles - pharmacology
Science & Technology
Sodium - metabolism
Vasoconstriction - drug effects
Veins - drug effects
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Summary:Ouabain (1×10-3×10 M) elicited concentration-dependent vasoconstriction in human placental arteries and veins. These responses, but not those produced by 10 M 5-hydroxytryptamine, were increased after the removal of vascular endothelium. In placental arteries, the respective blockade of cyclooxygenase or lipoxygenase with indomethacin or 5,8,11,15-eicosatetraynoic acid as well as the inactivation of nitric oxide with phenidone or oxyhemoglobin and the inhibition of nitric oxide synthesis with N-monomethyl l-arginine (all at 10 M) did not mimic the effects of endothelial denudation on ouabain-evoked contractions. Bioassay experiments suggested that the above-mentioned endothelial effects are mediated by diffusible factors. Rb uptake, a method to measure sodium pump activity, was significantly reduced by the removal of endothelium. These results suggest the existence of an inhibitory modulation by the endothelium of the contractions induced by ouabain, likely mediated by a diffusible factor(s) released from these cells. The nature of this substance is unknown but is not related to prostaglandins or leukotrienes, and neither is it a nitric oxide-related compound. Its mechanism of action could be stimulating the activity of vascular sodium pump and/or antagonizing its inhibition by ouabain.
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.71.4.943