The optimal temperature for RNA replication in cells infected by Soil-borne wheat mosaic virus is 17 degrees C

Systemic infection of wheat plants with Soil-borne wheat mosaic virus (SBWMV) requires temperatures below 20 degrees C. Here we examine the cause of the temperature sensitivity by inoculating infectious in vitro transcripts of SBWMV RNA1 and RNA2 to barley mesophyll protoplasts. After RNA inoculatio...

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Bibliographic Details
Published in:	Journal of general virology 2003-04, Vol.84 (Pt 4), p.995-1000
Main Authors:	Ohsato, Shuichi, Miyanishi, Masaki, Shirako, Yukio
Format:	Article
Language:	eng
Subjects:	Cells, Cultured Hordeum Mosaic Viruses - genetics Mosaic Viruses - physiology Mutation Protoplasts RNA Replicase - genetics RNA, Messenger - analysis RNA, Viral - biosynthesis Soil Microbiology Temperature Triticum - virology Virus Replication
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Summary:	Systemic infection of wheat plants with Soil-borne wheat mosaic virus (SBWMV) requires temperatures below 20 degrees C. Here we examine the cause of the temperature sensitivity by inoculating infectious in vitro transcripts of SBWMV RNA1 and RNA2 to barley mesophyll protoplasts. After RNA inoculation, protoplasts were incubated at temperatures between 15 and 25 degrees C for up to 48 h. Western blot analysis showed that the capsid protein accumulated most abundantly at 17 degrees C but was not detectable at 25 degrees C. Northern blot analysis showed that the wild-type RNA1 and RNA2 and their subgenomic RNAs accumulated most abundantly at 17 degrees C but were barely detectable at 25 degrees C. An RNA1 mutant in which the p152 and p211 replicase genes were placed between the 5'- and 3'-untranslated regions also replicated most efficiently at 17 degrees C but not at 25 degrees C. Thus, the requirement for temperatures lower than 20 degrees C for SBWMV infection is primarily determined by replication of RNA1, which encodes the viral RNA replicase.
ISSN:	0022-1317 1465-2099