Presence of endometrial epithelial cells in the peritoneal cavity and the mesothelial inflammatory response

To determine the contribution of endometrial cells in the development of endometriosis. Specifically the response of the mesothelium to endometrial cells in the production of monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), and IL-8 was studied. In vitro study. University Research Labor...

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Bibliographic Details
Published in:Fertility and sterility 2003-03, Vol.79, p.789-794
Main Authors: Song, Mingqing, Karabina, Sonia A, Kavtaradze, Nino, Murphy, Ana A, Parthasarathy, Sampath
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemokine CCL2 - biosynthesis
Chemokine CCL2 - genetics
Coculture Techniques
endometrial cells
endometriosis
Endometriosis - immunology
Endometriosis - metabolism
Endometriosis - pathology
Enzyme-Linked Immunosorbent Assay
Epithelial Cells - immunology
Epithelial Cells - metabolism
Epithelial Cells - pathology
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
inflammatory reaction
Interleukin-6 - biosynthesis
Interleukin-6 - genetics
Interleukin-8 - biosynthesis
Interleukin-8 - genetics
Medical sciences
Non tumoral diseases
Peritoneal mesothelial cells
Peritoneum - immunology
Peritoneum - metabolism
Peritoneum - pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA - chemistry
RNA - genetics
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Summary:To determine the contribution of endometrial cells in the development of endometriosis. Specifically the response of the mesothelium to endometrial cells in the production of monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), and IL-8 was studied. In vitro study. University Research Laboratory. None. None. Cellular MCP-1, IL-6 secretion and MCP-1, and IL-6 and IL-8 messenger RNA expression were evaluated by ELISA and reverse transcription-polymerase chain reaction (RT-PCR) assay. The mesothelial cells produced more MCP-1 and IL-6 than endometrial epithelial and stromal cells. Mesothelial cells cultured in the presence of endometrial epithelial cells produced even greater levels of MCP-1 and IL-6 than those cultured in the presence of stromal cells or cultured alone. The MCP-1, IL-6, and IL-8 mRNA expression also increased when mesothelial cells were co-cultured with endometrial epithelial cells. The results suggest that endometrial epithelial cells may be important in evoking the inflammatory reaction in the peritoneal cavity during retrograde menstruation and that mesothelial cells may play an important role in the chemotaxis of monocytes and in the inflammatory process during the development of endometriosis.
ISSN:0015-0282
1556-5653
DOI:10.1016/S0015-0282(02)04836-7