Infection of human fibroblast-like synovial cells with Chlamydia trachomatis results in persistent infection and interleukin-6 production

Recent studies have shown that the urogenital pathogen Chlamydia trachomatis to be a major bacterium triggering reactive arthritis (ReA), and is able to induce interleukin-6 (IL-6) production in human fibroblast-like synovial cells (FSC) in vitro. In the present study, we examined the correlation be...

Full description

Saved in:
Bibliographic Details
Published in:Microbial pathogenesis 2003-02, Vol.34 (2), p.57-63
Main Authors: Hanada, Hirofumi, Ikeda-Dantsuji, Yurika, Naito, Masatoshi, Nagayama, Ariaki
Format: Article
Language:English
Subjects:
Abnormal morphology
Adult
Azithromycin - pharmacology
Bacteriology
Biological and medical sciences
Chlamydia Infections - immunology
Chlamydia trachomatis
Chlamydia trachomatis - growth & development
Chlamydia trachomatis - physiology
Chlamydia trachomatis - radiation effects
Chlamydia trachomatis - ultrastructure
Female
Fibroblasts - cytology
Fibroblasts - microbiology
Fundamental and applied biological sciences. Psychology
HeLa Cells
Hot Temperature
Humans
Interleukin-6
Interleukin-6 - analysis
Interleukin-6 - biosynthesis
Male
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Persistence
Synovial cells
Synovial Membrane - cytology
Synovial Membrane - immunology
Synovial Membrane - microbiology
Ultraviolet Rays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recent studies have shown that the urogenital pathogen Chlamydia trachomatis to be a major bacterium triggering reactive arthritis (ReA), and is able to induce interleukin-6 (IL-6) production in human fibroblast-like synovial cells (FSC) in vitro. In the present study, we examined the correlation between IL-6 production and multiplication of chlamydia in FSC. All FSC from five patients secreted highly increased quantities of IL-6 in a dose-dependent and time-dependent fashion. Heat and UV inactivated chlamydia failed to enhance production of IL-6. When azithromycin was added to infected cultures of FSC at 0 or 48 h after infection, the level of IL-6 production was very low. Transmission electron microscopy of such infected cultures revealed many abnormal forms of chlamydia within the inclusions in FSC. From one step-growth curve experiments, it was suggested that C. trachomatis hardly multiplied in FSC. In contrast, in C. trachomatis infected HeLa 229 cells, chlamydia multiplied as usual, but little IL-6 production were found. These observations indicated that live chlamydia and the persistence of chlamydia may be essential for stimulating the synthesis of IL-6 in FSC.
ISSN:0882-4010
1096-1208
DOI:10.1016/S0882-4010(02)00189-4