Increased expression of a scavenger receptor (CD36) in monocytes from subjects with Type 2 diabetes

Uptake of modified low density lipoprotein (LDL) by monocyte-macrophages is mediated by the scavenger receptor CD36, which is upregulated in vitro by high glucose concentrations and oxidatively modified LDL. We hypothesised that monocyte CD36 expression would be higher in Type 2 diabetes, and would...

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Bibliographic Details
Published in:Atherosclerosis 2003-03, Vol.167 (1), p.129-134
Main Authors: SAMPSON, Michael J, DAVIES, Isabel R, BRASCHI, Simon, IVORY, Kamal, HUGHES, David A
Format: Article
Language:English
Subjects:
Adult
Aged
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Blood Glucose - analysis
Cardiology. Vascular system
Case-Control Studies
CD36 Antigens - genetics
CD36 Antigens - metabolism
Cohort Studies
Diabetes Mellitus, Type 2 - genetics
Diabetes Mellitus, Type 2 - physiopathology
Female
Gene Expression Regulation
Glucose Tolerance Test
Humans
Lipoproteins, LDL - genetics
Lipoproteins, LDL - metabolism
Male
Medical sciences
Membrane Proteins
Middle Aged
Monocytes
Probability
Receptors, Immunologic - genetics
Receptors, Immunologic - metabolism
Receptors, Lipoprotein
Receptors, Scavenger
Reference Values
RNA, Messenger - analysis
Scavenger Receptors, Class B
Sensitivity and Specificity
Up-Regulation - genetics
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Summary:Uptake of modified low density lipoprotein (LDL) by monocyte-macrophages is mediated by the scavenger receptor CD36, which is upregulated in vitro by high glucose concentrations and oxidatively modified LDL. We hypothesised that monocyte CD36 expression would be higher in Type 2 diabetes, and would increase during acute hyperglycaemia. Sixteen subjects with Type 2 diabetes and 11 controls underwent a 75 g oral glucose load. Monocyte CD36 expression (by laser flow cytometry), plasma LDL diene conjugates, plasma LDL hydroxyoctadecadienoic acid-13 (a peroxisome proliferator activator receptor gamma agonist) were measured at 0, 2 and 4 h. Mean monocyte CD36 expression at baseline was 34% higher in the diabetes group (P=0.01), did not change during acute hyperglycaemia and plasma LDL conjugated diene concentration was the only variable directly related to CD36 expression (F=4.53; P=0.05; r=0.51). Higher baseline CD36 expression in Type 2 diabetes could reflect increased post-transcriptional efficiency of CD36 mRNA in response to chronic hyperglycaemia and could be a proatherogenic mechanism in Type 2 diabetes.
ISSN:0021-9150
1879-1484
DOI:10.1016/S0021915002004215