Membrane peroxidation by lipopolysaccharide and iron-ascorbate adversely affects Caco-2 cell function: beneficial role of butyric acid

Membrane lipid peroxidation may play a role in immune-mediated bowel diseases. We examined the effects of lipopolysaccharide (LPS), a ubiquitous endotoxin mediator of gram-negative bacteria, alone and in combination with iron-ascorbate, on enterocyte function. Furthermore, we assessed the antioxidan...

Full description

Saved in:
Bibliographic Details
Published in:The American journal of clinical nutrition 2003-03, Vol.77 (3), p.744-750
Main Authors: COURTOIS, Frederic, SEIDMAN, Ernest G, DELVIN, Edgard, ASSELIN, Claude, BERNOTTI, Sandra, LEDOUX, Marielle, LEVY, Emile
Format: Article
Language:English
Subjects:
Antioxidants
Antioxidants - pharmacology
Ascorbic Acid - pharmacology
Bacterial diseases
Biological and medical sciences
Butylated Hydroxytoluene - pharmacology
Butyric Acid - pharmacology
Caco-2 Cells - metabolism
Caco-2 Cells - physiology
Cell Membrane Permeability - drug effects
Cell Survival - drug effects
Clinical trials
Cyclooxygenase 2
Dinoprostone - biosynthesis
Enterocytes - physiology
Experimental bacterial diseases and models
Humans
Infectious diseases
Iron
Isoenzymes - biosynthesis
Lipid Peroxidation - drug effects
Lipopolysaccharides - pharmacology
Malondialdehyde - analysis
Medical sciences
Membrane Fluidity - drug effects
Membrane Lipids - metabolism
Membrane Proteins
Oxidation-Reduction
Prostaglandin-Endoperoxide Synthases - biosynthesis
Sucrase - antagonists & inhibitors
Sucrase - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Membrane lipid peroxidation may play a role in immune-mediated bowel diseases. We examined the effects of lipopolysaccharide (LPS), a ubiquitous endotoxin mediator of gram-negative bacteria, alone and in combination with iron-ascorbate, on enterocyte function. Furthermore, we assessed the antioxidant capacity of butylated hydroxytoluene (BHT) and butyric acid, which are known to play a significant role in the welfare of intestinal mucosa. Differentiated intestinal Caco-2 cells were used to study the induction of membrane peroxidation by LPS (100 micro g/mL) and iron-ascorbate (0.2 and 2 mmol/L, respectively) and to examine the beneficial effects of BHT and butyric acid. A significant dose-dependent increase in malondialdehyde, accompanied by lower apical membrane fluidity and significantly decreased sucrase activity, was observed when Caco-2 cells were incubated with LPS. LPS also augmented paracellular permeability ([(14)C]polyethylene glycol flux), prostaglandin E(2) production, and cyclooxygenase-2 (EC 1.14.99.1) expression. These abnormalities were exacerbated by the coadministration of iron-ascorbate, but most of them were suppressed by butyric acid and BHT. Bacterial endotoxin and prooxidants may overwhelm antioxidant defenses and become deleterious to enterocyte function, whereas butyric acid and BHT may provide antioxidant protection.
ISSN:0002-9165
1938-3207
DOI:10.1093/ajcn/77.3.744