COX16 Encodes a Novel Protein Required for the Assembly of Cytochrome Oxidase in Saccharomyces cerevisiae

We have characterized Cox16p, a new cytochrome oxidase (COX) assembly factor. This protein is encoded byCOX16, corresponding to the previously uncharacterized open reading frame YJL003w of the yeast genome.COX16 was identified in studies of COX-deficient mutants previously assigned to complementatio...

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Bibliographic Details
Published in:The Journal of biological chemistry 2003-02, Vol.278 (6), p.3770-3775
Main Authors: Carlson, Christopher G., Barrientos, Antoni, Tzagoloff, Alexander, Glerum, D. Moira
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cloning, Molecular
Electron Transport Complex IV - metabolism
Humans
Membrane Proteins
Mice
Mitochondria - enzymology
Mitochondrial Proteins - chemistry
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
Molecular Sequence Data
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae Proteins - chemistry
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Sequence Homology, Amino Acid
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Summary:We have characterized Cox16p, a new cytochrome oxidase (COX) assembly factor. This protein is encoded byCOX16, corresponding to the previously uncharacterized open reading frame YJL003w of the yeast genome.COX16 was identified in studies of COX-deficient mutants previously assigned to complementation group G22 of a collection of yeast pet mutants. To determine its location, Cox16p was tagged with a Myc epitope at the C terminus. The fusion protein, when expressed from a low-copy plasmid, complements the mutant and is detected solely in mitochondria. Cox16p-myc is an integral component of the mitochondrial inner membrane, with its C terminus exposed to the intermembrane space. Cox16 homologues are found in both the human and murine genomes, although human COX16 does not complement the yeast mutant. Cox16p does not appear to be involved in maturation of subunit 2, copper recruitment, or heme A biosynthesis. Cox16p is thus a new protein in the growing family of eukaryotic COX assembly factors for which there are as yet no specific functions known. Like other recently described nuclear gene products involved in expression of cytochrome oxidase, COX16 is a candidate for screening in inherited human COX deficiencies.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M209893200