Molecular cloning and characterisation of peroxisome proliferator activated receptor gamma1 (PPARγ1) cDNA gene from guinea pig ( Cavia porcellus): tissue distribution

The coding region of guinea pig peroxisome proliferator activated receptor gamma1 (gpPPARγ1) cDNA was successfully cloned from adipose tissue by reverse transcription polymerase chain reaction (RT-PCR) using the designated primers based on the conserved regions of the other mammalian PPARγ1 sequence...

Full description

Saved in:
Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2003, Vol.134 (1), p.37-44
Main Authors: Khoo, B.Y, Samian, M.R, Najimudin, N, Tengku Muhammad, T.S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Cell Nucleus - metabolism
Cloning
Cloning, Molecular
DNA, Complementary - metabolism
Electrophoresis, Polyacrylamide Gel
Gene expression
Guinea pig
Guinea Pigs
Male
Models, Genetic
Molecular Sequence Data
Nuclear hormone receptor
Phylogeny
PPARγ
Receptors, Cytoplasmic and Nuclear - biosynthesis
Receptors, Cytoplasmic and Nuclear - chemistry
Receptors, Cytoplasmic and Nuclear - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
RT-PCR
Sequencing
Tissue Distribution
Transcription factor
Transcription Factors - biosynthesis
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
Western blot analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The coding region of guinea pig peroxisome proliferator activated receptor gamma1 (gpPPARγ1) cDNA was successfully cloned from adipose tissue by reverse transcription polymerase chain reaction (RT-PCR) using the designated primers based on the conserved regions of the other mammalian PPARγ1 sequence. From RT-PCR, a combination of three cDNA fragments that comprised of the full length coding region PPARγ1 cDNA gene were amplified, with the size of 498, 550 and 557 bp, respectively. All three fragments were then successfully assembled by utilising the internal restriction sites present at the overlapping regions to give rise to the full-length coding region of gpPPARγ1 with the size of 1428 bp and consisting of 475 amino acids. Guinea pig PPARγ1 is highly conserved with those of other species at protein and nucleotide levels. Gene expression studies showed that gpPPARγ mRNA was predominantly expressed in adipose tissue followed by lung and spleen. However, at the protein level, PPARγ was also found to be expressed in skeletal muscle.
ISSN:1096-4959
1879-1107
DOI:10.1016/S1096-4959(02)00219-1