Mass spectrometric analysis of the perisympathetic organs in locusts: identification of novel periviscerokinins

A mass spectrometric analysis carried out to determine the peptidome of the abdominal perisympathetic organs in the locust species Locusta migratoria and Schistocerca gregaria yielded a number of predominant ion peaks, among which are Lom-PVK (AAGLFQFPRVamide) and Scg-MT-2 (TSSLFPHPRLamide). In addi...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-01, Vol.300 (2), p.422-428
Main Authors: Clynen, Elke, Huybrechts, Jurgen, De Loof, Arnold, Schoofs, Liliane
Format: Article
Language:English
Subjects:
Abdominal Cavity
Amino Acid Sequence
Animals
Capability
Grasshoppers - chemistry
Locusta migratoria
Mass Spectrometry
Molecular Sequence Data
Nervous System - chemistry
Neuropeptides
Neuropeptides - analysis
Neuropeptides - chemistry
Perisympathetic organ
Periviscerokinin
Pyrokinin
Schistocerca gregaria
Sympathetic Nervous System - chemistry
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Summary:A mass spectrometric analysis carried out to determine the peptidome of the abdominal perisympathetic organs in the locust species Locusta migratoria and Schistocerca gregaria yielded a number of predominant ion peaks, among which are Lom-PVK (AAGLFQFPRVamide) and Scg-MT-2 (TSSLFPHPRLamide). In addition, three novel peptides were identified: Lom-PVK-2 (identical in Schistocerca): GLLAFPRVamide, Lom-PVK-3: DGGEPAAPLWFGPRVamide, and Scg-PVK-3: DGAETPGAAASLWFGPRVamide. An extensive mass spectrometric study of the central nervous system showed that the periviscerokinins (-PRVamides) and Scg-MT-2 (-FXXPRLamide) are restricted to the abdominal ganglia and their perisympathetic organs, while the pyrokinins (-FXPRLamides) are present only in the brain–retrocerebral complex. Sequence comparison with the Drosophila genes supports a conserved gene structure whereby a capability-like gene encodes the periviscerokinins that are expressed in the abdominal ganglia and stored in the perisympathetic organs, while a hugin-like gene encodes the pyrokinins that are expressed in the head ganglia and stored in the retrocerebral complex.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)02869-3