Automated Video Microscopic Imaging and Data Acquisition System for Colloid Deposition Measurements

An optical video microscopic system and image processing and data extraction and manipulation routines are developed for in situ detailed quantification of the deposition of colloids onto an arbitrary surface and determining their concentration distribution across the bulk suspension. The system pro...

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Bibliographic Details
Published in:Journal of colloid and interface science 2002-02, Vol.246 (2), p.241-258
Main Authors: Abdel-Fattah, Amr I., El-Genk, Mohamed S., Reimus, Paul W.
Format: Article
Language:English
Subjects:
arrival flux
attachment
Chemistry
colloid deposition
Colloidal state and disperse state
colloids
connectivity
dark field
departure flux
detachment
Exact sciences and technology
General and physical chemistry
image processing
parallel plate geometry
Physical and chemical studies. Granulometry. Electrokinetic phenomena
residence time
videomicroscopy
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Description
Summary:An optical video microscopic system and image processing and data extraction and manipulation routines are developed for in situ detailed quantification of the deposition of colloids onto an arbitrary surface and determining their concentration distribution across the bulk suspension. The system produces a relatively large field of view (∼330×245μm) and utilizes dark-field light microscopy to visualize colloids as small as ∼0.3μm in diameter at the surface and in the bulk suspension with a sufficient resolution (∼0.5μm). On real-time basis, the routines automate various tasks from image capturing and processing to data manipulation, extraction, and display. The extracted data include: (i) surface concentration and flux of deposited, attached, and detached colloids, (ii) surface concentration and flux of arriving and departing colloids, (iii) distribution of colloids in the bulk suspension in the direction perpendicular to the deposition surface, and (iv) spatial and temporal distributions of deposited colloids. This article provides detailed description of the system and its image processing and data extraction and manipulation routines. Representative results of the deposition of 0.3-μm-diameter polystyrene colloids onto a glass surface, from a flowing suspension in a 0.02-cm-aperture parallel-plate channel, are presented and discussed.
ISSN:0021-9797
1095-7103
DOI:10.1006/jcis.2001.7948