Assessment of population genetic structure in common wild rice Oryza rufipogon Griff. using microsatellite and allozyme markers

The genetic structure of five natural populations of common wild rice Oryza rufipogon Griff. from China, was investigated with 21 microsatellite loci and compared to estimates of genetic diversity and genetic differentiation detected by 22 allozyme loci. Microsatellite loci, as expected, have much h...

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Bibliographic Details
Published in:Theoretical and applied genetics 2002-12, Vol.106 (1), p.173-180
Main Authors: GAO, L-Z, SCHAAL, B. A, ZHANG, C-H, JIA, J-Z, DONE, Y-S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Edible Grain - enzymology
Edible Grain - genetics
Fundamental and applied biological sciences. Psychology
Gene Frequency
Genetic aspects
Genetic diversity
Genetic Markers
Genetic Variation
Genetics of eukaryotes. Biological and molecular evolution
Genetics, Population
Genomes
Germplasm
Isoenzymes - genetics
Microsatellite Repeats - genetics
Microsatellites (Genetics)
Observations
Phylogeny
Plant genetics
Polymorphism, Genetic
Population biology
Population genetics
Population genetics, reproduction patterns
Pteridophyta, spermatophyta
Rice
Vegetals
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Summary:The genetic structure of five natural populations of common wild rice Oryza rufipogon Griff. from China, was investigated with 21 microsatellite loci and compared to estimates of genetic diversity and genetic differentiation detected by 22 allozyme loci. Microsatellite loci, as expected, have much higher levels of genetic diversity (mean values of A = 3.1, P = 73.3%, Ho = 0.358 and He = 0.345) than allozyme loci (mean values of A = 1.2, P = 12.7%, Ho = 0.020 and He = 0.030). Genetic differentiation detected by microsatellite loci ( FST = 0.468, mean I = 0.472) was higher than that for allozyme loci ( FST =0.388, mean I = 0.976). However, microsatellite markers showed less deviation from Hardy-Weinberg expectation (Wright's inbreeding coefficient FIS = -0.069) than do allozymes ( FIS = 0.337). These results suggest that microsatellite markers are powerful high-resolution tools for the accurate assessment of important parameters in population biology and conservation genetics of O. rufipogon, and offer advantages over allozyme markers.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-002-1027-9