Different profile of CD8+ effector T cells induced in Der p 1‐allergic and naïve mice by DNA vaccination

DNA vaccination holds great promise in both prophylactic and therapeutic vaccines. Recent evidence suggests that DNA vaccines could be powerful therapies countering Th2‐mediated disorders suchas allergies. Here, we studied the allergen‐specific CD4+ and CD8+ T cell populations induced following immu...

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Bibliographic Details
Published in:European journal of immunology 2002-12, Vol.32 (12), p.3720-3728
Main Authors: Draghi, Monia, Jarman, Elizabeth R., Grifantini, Renata, Galli‐Stampino, Luisa, Lamb, Jonathan R., Valiante, Nicholas M., Grandi, Guido
Format: Article
Language:English
Subjects:
Allergens - administration & dosage
Allergens - genetics
Allergens - immunology
Animals
Antigens, Dermatophagoides - administration & dosage
Antigens, Dermatophagoides - genetics
Antigens, Dermatophagoides - immunology
Arthropod Proteins
Base Sequence
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cysteine Endopeptidases
Cytolitic activity
Cytotoxicity, Immunologic
Der p 1
DNA vaccination
Epitopes - administration & dosage
Epitopes - genetics
Female
Hypersensitivity - immunology
Hypersensitivity - therapy
Immunization
Interferon-gamma - biosynthesis
Mice
Mice, Inbred C57BL
T-Lymphocytes - immunology
Th1 Cells - immunology
Th1/Th2 response
Th2 Cells - immunology
Vaccines, DNA - genetics
Vaccines, DNA - pharmacology
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Summary:DNA vaccination holds great promise in both prophylactic and therapeutic vaccines. Recent evidence suggests that DNA vaccines could be powerful therapies countering Th2‐mediated disorders suchas allergies. Here, we studied the allergen‐specific CD4+ and CD8+ T cell populations induced following immunization of allergic and non‐allergic mice with DNA vaccine vectors encoding discrete epitopes of the house dust mite (HDM) Dermatophagoides pteronyssinus group I (Der p 1) allergen. Specifically, mice were sensitized to Der p 1 and exhibited a strong Th2/allergic response. Sensitized and non‐allergic mice were then compared for their responses to DNA immunization. Using Elispot analysis, we demonstrate that allergic/vaccinated mice generate a mixed Th1/Th2 response against the allergen with high numbers of allergen‐specific CD4+ T cells secreting IFN‐γ or IL‐4, whereas in non‐allergic/vaccinated mice a polarized Th1 response was dominant. Allergen‐specific CD8+ T cells secreting IFN‐γ were induced at equal frequencies in both allergic and non‐allergic mice. However, the CD8+ T cells from allergic mice were markedly deficient in their cytotoxic potential when compared to their counterparts in non‐allergic mice. These results indicate that during an ongoing Th2 response, DNA vaccination leads to the generation of a distinct population of non‐cytotoxic/regulatory CD8+ T cells.
ISSN:0014-2980
1521-4141
DOI:10.1002/1521-4141(200212)32:12<3720::AID-IMMU3720>3.0.CO;2-J