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Different profile of CD8+ effector T cells induced in Der p 1‐allergic and naïve mice by DNA vaccination
DNA vaccination holds great promise in both prophylactic and therapeutic vaccines. Recent evidence suggests that DNA vaccines could be powerful therapies countering Th2‐mediated disorders suchas allergies. Here, we studied the allergen‐specific CD4+ and CD8+ T cell populations induced following immu...
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Published in: | European journal of immunology 2002-12, Vol.32 (12), p.3720-3728 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | DNA vaccination holds great promise in both prophylactic and therapeutic vaccines. Recent evidence suggests that DNA vaccines could be powerful therapies countering Th2‐mediated disorders suchas allergies. Here, we studied the allergen‐specific CD4+ and CD8+ T cell populations induced following immunization of allergic and non‐allergic mice with DNA vaccine vectors encoding discrete epitopes of the house dust mite (HDM) Dermatophagoides pteronyssinus group I (Der p 1) allergen. Specifically, mice were sensitized to Der p 1 and exhibited a strong Th2/allergic response. Sensitized and non‐allergic mice were then compared for their responses to DNA immunization. Using Elispot analysis, we demonstrate that allergic/vaccinated mice generate a mixed Th1/Th2 response against the allergen with high numbers of allergen‐specific CD4+ T cells secreting IFN‐γ or IL‐4, whereas in non‐allergic/vaccinated mice a polarized Th1 response was dominant. Allergen‐specific CD8+ T cells secreting IFN‐γ were induced at equal frequencies in both allergic and non‐allergic mice. However, the CD8+ T cells from allergic mice were markedly deficient in their cytotoxic potential when compared to their counterparts in non‐allergic mice. These results indicate that during an ongoing Th2 response, DNA vaccination leads to the generation of a distinct population of non‐cytotoxic/regulatory CD8+ T cells. |
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ISSN: | 0014-2980 1521-4141 |
DOI: | 10.1002/1521-4141(200212)32:12<3720::AID-IMMU3720>3.0.CO;2-J |