Isolation of mononuclear cytotoxic cells from cattle vaccinated against foot-and-mouth disease

Mononuclear cells from peripheral blood leucocytes of foot-and-mouth disease (FMD) vaccinated cattle underwent blast transformation after in vitro culture with purified, inactivated, 146 S FMD virus antigen. From the prolonged culture of blast cells in medium with Interleukin-2 (5-10 U/ml), CD 45-po...

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Bibliographic Details
Published in:Archives of virology 1992-01, Vol.122 (3/4), p.293-306
Main Authors: Amadori, M, Archetti, I.L, Verardi, R, Berneri, C
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Aphthovirus
Aphthovirus - immunology
Biological and medical sciences
Cattle
Cell Separation
cell-mediated cytotoxicity
Cells, Cultured
cytotoxic T-lymphocytes
cytotoxicity
foot-and-mouth disease
foot-and-mouth disease virus
Fundamental and applied biological sciences. Psychology
immunity
Killer Cells, Natural - immunology
Life Sciences & Biomedicine
live vaccines
Lymphocyte Activation
Microbiology
Receptors, Fc - metabolism
replication
Science & Technology
T-Lymphocytes, Cytotoxic - immunology
T-Lymphocytes, Cytotoxic - metabolism
vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Attenuated
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Virology
Virus Replication
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Summary:Mononuclear cells from peripheral blood leucocytes of foot-and-mouth disease (FMD) vaccinated cattle underwent blast transformation after in vitro culture with purified, inactivated, 146 S FMD virus antigen. From the prolonged culture of blast cells in medium with Interleukin-2 (5-10 U/ml), CD 45-positive effector cells were derived, which showed a potent, non MHC-restricted activity against virus-infected cells, the extent of which was inversely correlated with multiplicity of infection (MOI). Extensive characterization of effector cells by means of monoclonal antibodies (MAbs) in flow cytometry, lysis of various cell populations with MAbs and complement, Fc receptor analysis and 51Cr release assays indicated that the isolated cells share some phenotypic and functional features of the human and murine Large Granular Lymphocyte/natural Killer (N.K.) lineage.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01317191