Stoichiometry of the Photosynthetic Apparatus and Phycobilisome Structure of the Cyanobacterium Plectonema boryanum UTEX 485 Are Regulated by Both Light and Temperature

The role of growth temperature and growth irradiance on the regulation of the stoichiometry and function of the photosynthetic apparatus was examined in the cyanobacterium Plectonema boryanum UTEX 485 by comparing mid-log phase cultures grown at either 29°C/150 μmol m-2 s-1, 29°C/750 μmol m-2 s-1, 1...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 2002-11, Vol.130 (3), p.1414-1425
Main Authors: Miskiewicz, Ewa, Ivanov, Alexander G, Huner, Norman P A
Format: Article
Language:English
Subjects:
Acclimatization
Acclimatization - physiology
Acclimatization - radiation effects
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Bacterial Proteins - radiation effects
Cell Biology and Signal Transduction
Cell growth
Chlorophyll - antagonists & inhibitors
Chlorophyll - metabolism
Cyanobacteria
Cyanobacteria - physiology
Cyanobacteria - radiation effects
Cyanophyta
Cytochrome b Group - drug effects
Cytochrome b Group - metabolism
Cytochrome b6f Complex
Cytochromes
Cytochromes - drug effects
Cytochromes - metabolism
Cytochromes f
Electron Transport - physiology
Enzyme Inhibitors - pharmacology
Fluorescence
Irradiance
Light
Light-Harvesting Protein Complexes
Low temperature
NADPH Dehydrogenase - drug effects
NADPH Dehydrogenase - metabolism
Oxidation-Reduction - drug effects
Photosynthetic Reaction Center Complex Proteins - chemistry
Photosynthetic Reaction Center Complex Proteins - metabolism
Photosynthetic Reaction Center Complex Proteins - radiation effects
Phycobilisomes
Physiological regulation
Plants
Plastoquinone - antagonists & inhibitors
Plastoquinone - metabolism
Plectonema boryanum
Proteins - chemistry
Proteins - metabolism
Proteins - radiation effects
Signal Transduction - drug effects
Signal Transduction - radiation effects
Stoichiometry
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The role of growth temperature and growth irradiance on the regulation of the stoichiometry and function of the photosynthetic apparatus was examined in the cyanobacterium Plectonema boryanum UTEX 485 by comparing mid-log phase cultures grown at either 29°C/150 μmol m-2 s-1, 29°C/750 μmol m-2 s-1, 15°C/150 μmol m-2 s-1, or 15°C/10 μmol m-2 s-1. Cultures grown at 29°C/750 μmol m-2 s-1 were structurally and functionally similar to those grown at 15°C/150 μmol m-2 s-1, whereas cultures grown at 29°C/150 μmol m-2 s-1 were structurally and functionally similar to those grown at 15°C/10 μmol m-2 s-1. The stoichiometry of specific components of the photosynthetic apparatus, such as the ratio of photosystem (PS) I to PSII, phycobilisome size and the relative abundance of the cytochrome $\text{b}_{6}$/f complex, the plastoquinone pool size, and the NAD(P)H dehydrogenase complex were regulated by both growth temperature and growth irradiance in a similar manner. This indicates that temperature and irradiance may share a common sensing/signaling pathway to regulate the stoichiometry and function of the photosynthetic apparatus in P. boryanum. In contrast, the accumulation of neither the D1 polypeptide of PSII, the large subunit of Rubisco, nor the CF1 α-subunit appeared to be regulated by the same mechanism. Measurements of P700 photooxidation in vivo in the presence and absence of inhibitors of photosynthetic electron transport coupled with immunoblots of the NAD(P)H dehydrogenase complex in cells grown at either 29°C/750 μmol m-2 s-1 or 15°C/150 μmol m-2 s-1 are consistent with an increased flow of respiratory electrons into the photosynthetic intersystem electron transport chain maintaining P700 in a reduced state relative to cells grown at either 29°C/150 μmol m-2 s-1 or 15°C/10 μmol m-2 s-1. These results are discussed in terms of acclimation to excitation pressure imposed by either low growth temperature or high growth irradiance.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.008631