Acute inhibition of glutathione biosynthesis alters endothelial function and blood pressure in rats

The cardiovascular and biochemical responses during acute oxidative stress induced by d, l-buthionine-( S, R)-sulfoximine (BSO) were investigated in Sprague–Dawley rats. Mean arterial pressure, heart rate and vascular reactivity were measured after subcutaneous injection of BSO (4 mmol/kg). Control...

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Published in:European journal of pharmacology 2002-11, Vol.454 (2), p.217-223
Main Authors: Ganafa, Agaba A, Socci, Robin R, Eatman, Danita, Silvestrova, Natalia, Abukhalaf, Imad K, Bayorh, Mohamed A
Format: Article
Language:English
Subjects:
Animals
Blood pressure
Blood Pressure - drug effects
Blood Pressure - physiology
Buthionine Sulfoximine - pharmacology
Dose-Response Relationship, Drug
Endothelial function
Endothelium, Vascular - drug effects
Endothelium, Vascular - metabolism
Glutathione
Glutathione - antagonists & inhibitors
Glutathione - biosynthesis
Male
Nitric Oxide - metabolism
Oxidative stress
Oxidative Stress - drug effects
Oxidative Stress - physiology
Rats
Rats, Sprague-Dawley
Space life sciences
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Summary:The cardiovascular and biochemical responses during acute oxidative stress induced by d, l-buthionine-( S, R)-sulfoximine (BSO) were investigated in Sprague–Dawley rats. Mean arterial pressure, heart rate and vascular reactivity were measured after subcutaneous injection of BSO (4 mmol/kg). Control rats received saline. Levels of GSH and GSSG in blood and tissues as well as renal superoxide were determined. Nitric oxide, prostacyclin and thromboxane A 2 in plasma and aorta, and isoprostane in plasma were also measured. Blood pressure was elevated at 24 h (121±2 vs. 104±2 mm Hg), with increased reactivity to phenylephrine (by a 59±4 vs. 45±2 mm Hg change), and impaired response to sodium nitroprusside (by a −35±2 vs. −63±2 mm Hg change), P
ISSN:0014-2999
1879-0712
DOI:10.1016/S0014-2999(02)02500-1