A truncated mutant of the extrinsic 23-kDa protein that absolutely requires the extrinsic 17-kDa protein for Ca2+ retention in photosystem II

One function of the extrinsic 23-kDa protein in photosystem II (OEC23) is to retain Ca2+ and Cl–, two essential cofactors for photosynthetic oxygen evolution. A truncated mutant of OEC23 (OEC23 Δ19) revealed that 19 residues of the N-terminus of OEC23 were necessary for Ca2+ retention but not for it...

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Bibliographic Details
Published in:Plant and cell physiology 2002-10, Vol.43 (10), p.1244-1249
Main Authors: Ifuku, K. (Kyoto Univ. (Japan)), Sato, F
Format: Article
Language:English
Subjects:
BINDING PROTEINS
BIOCHEMICAL REACTIONS
Calcium - metabolism
CALCIUM BINDING PROTEINS
CBB
Coomassie brilliant blue
INDUCED MUTATION
Keywords: Ca2+ retention — Extrinsic 23-kDa protein — Extrinsic 17-kDa protein — Oxygen-evolving complex — Photosystem II
Mutation
OEC17
OEC23
Oxygen - metabolism
PHOTOSYNTHESIS
Photosynthetic Reaction Center Complex Proteins - metabolism
Photosystem II Protein Complex
PHOTOSYSTEMS
Plant Proteins - genetics
Plant Proteins - metabolism
the extrinsic 23-kDa protein in an oxygen-evolving complex
the extrinsic17-kDa protein in an oxygen-evolving complex
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Summary:One function of the extrinsic 23-kDa protein in photosystem II (OEC23) is to retain Ca2+ and Cl–, two essential cofactors for photosynthetic oxygen evolution. A truncated mutant of OEC23 (OEC23 Δ19) revealed that 19 residues of the N-terminus of OEC23 were necessary for Ca2+ retention but not for its proper interaction with OEC17, the extrinsic 17-kDa protein in photosystem II. The lost ability of OEC23 Δ19 to reconstitute the oxygen-evolving activity was partially restored by OEC17 binding, suggesting the involvement of OEC17 in Ca2+ retention in photosystem II.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcf136