Effects of scrotal insulation on viability characteristics of cryopreserved bovine semen

The effect of a 48-h scrotal insulation on spermatozoal viability (motility and acrosomal integrity), before and after semen cryopreservation, was studied in six young Holstein bulls whose semen was collected twice in succession at 3-d intervals. Motility and acrosomal integrity were measured before...

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Bibliographic Details
Published in:Journal of dairy science 1991-11, Vol.74 (11), p.3827-3835
Main Authors: Vogler, C. J, Saacke, R. G, Bame, J. H, Dejarnette, J. M, McGilliard, M. L
Format: Article
Language:English
Subjects:
Acrosome - ultrastructure
agotamiento por el calor
aislamiento
Animals
Biological and medical sciences
bovin laitier
breeds
bulls
Cattle - physiology
congelacion
congelation
conservacion del semen
conservation du sperme
coup de chaleur
Cryopreservation
dairy cattle
escroto
espermatozoo
freezing
Fundamental and applied biological sciences. Psychology
ganado de leche
General aspects
heat exhaustion
Hot Temperature - adverse effects
insulation
isolation
Male
preservacion
preservation
race
razas
scrotum
semen
Semen Preservation
Sperm Count - veterinary
Sperm Motility
spermatozoa
Spermatozoa - physiology
spermatozoide
sperme
taureau
toro
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:The effect of a 48-h scrotal insulation on spermatozoal viability (motility and acrosomal integrity), before and after semen cryopreservation, was studied in six young Holstein bulls whose semen was collected twice in succession at 3-d intervals. Motility and acrosomal integrity were measured before and after incubation of semen at 37 degrees C for 3 h. For assessment of results, collection days were grouped: period 1 (control) = d -6, -3, and 0, where d 0 = initiation of scrotal insulation after semen collection; period 2 = d 3, 6, and 9 sperm presumed in the epididymis or rete testis during scrotal insulation); period 3 = d 12, 15, 39 (sperm presumed in spermatogenesis during scrotal insulation). Semen was cryopreserved each collection day until morphologically abnormal cells exceeded 50% of the ejaculate (d 12 to 21). Semen viability before and after freezing was lower in period 3 than in period 1 (P < 0.05). These differences coincided with the appearance in period 3 of abnormal sperm morphology and depressed undiluted semen motility, which began on d 12 (P < 0.01). Semen collected during period 2 that was extended but unfrozen did not differ from that collected during period 1 in morphology or viability. However, for frozen semen, period 2 was significantly poorer than period 1 for both viability measurements, but only after incubation for 3 h at 37 degrees C postthaw (P < 0.05). We conclude that epididymal sperm are adversely affected by elevated testicular temperatures, as noted by their decreased ability to maintain motility and acrosomal integrity following cryopreservation.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(91)78575-5