IL-12, IFN-γ, and TNF-α Released from Mononuclear Cells Inhibit the Spread of Varicella-Zoster Virus at an Early Stage of Varicella

The activity of mononuclear cells to inhibit plaque formation of varicella-zoster virus (VZV) was investigated by an in vitro infectious center assay. Peripheral blood mononuclear cells (PBMC) inhibited VZV plaque formation by co-cultivation with VZV-infected fibroblasts. As compared to mononuclear...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 2000, Vol.44(12), pp.1027-1031
Main Authors: Torigoe, Sadayoshi, Ihara, Toshiaki, Kamiya, Hitoshi
Format: Article
Language:English
Subjects:
Adult
Anti-Inflammatory Agents, Non-Steroidal - therapeutic use
Biological and medical sciences
Cells, Cultured
Chickenpox - blood
Chickenpox - drug therapy
Chickenpox - immunology
Chickenpox - prevention & control
Child
Coculture Techniques
Fibroblasts - cytology
Fundamental and applied biological sciences. Psychology
g-Interferon
Glucocorticoids - therapeutic use
Herpesvirus 3, Human - immunology
Herpesvirus 3, Human - pathogenicity
Humans
IFN-γ
IL-12
Infectious center assay
Interferon-alpha - biosynthesis
Interferon-gamma - biosynthesis
Interferon-gamma - immunology
Interleukin-12 - biosynthesis
Interleukin-12 - immunology
Interleukin-2 - biosynthesis
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - virology
Microbiology
Middle Aged
Prednisolone - therapeutic use
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Time Factors
Tumor Necrosis Factor-alpha - biosynthesis
Tumor Necrosis Factor-alpha - immunology
Varicella-zoster virus
Varicella-zoster virus 1
Viral Plaque Assay
Virology
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Summary:The activity of mononuclear cells to inhibit plaque formation of varicella-zoster virus (VZV) was investigated by an in vitro infectious center assay. Peripheral blood mononuclear cells (PBMC) inhibited VZV plaque formation by co-cultivation with VZV-infected fibroblasts. As compared to mononuclear cells from normal individuals, mononuclear cells from umbilical cord blood and from patients receiving corticosteroids showed a significant decrease in the ability to inhibit viral replication. This ability was significantly increased for mononuclear cells collected during the acute phase of varicella. PBMC obtained from patients in the acute phase of varicella produced significantly higher amounts of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and interleukin (IL)-12 in the supernatant compared with those of healthy individuals. These data suggest that the cytokines have an important role in the inhibition of the spread of VZV at an early stage of varicella. Th1 type adaptive immunity might play a major role in VZV infection.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2000.tb02599.x