Tumor necrosis factor-α and nitrite/nitrate responses during acute mastitis induced by Escherichia coli infection and endotoxin in dairy cows

Concentrations of tumor necrosis factor-α (TNF-α) and of NO x (sum of nitrite and nitrate as indicators of endogenous nitric oxide production) in milk and blood plasma were measured in three mastitis models in dairy cows in early lactation. Escherichia coli P4:037 bacteria or endotoxin 0111:B4 were...

Full description

Saved in:
Bibliographic Details
Published in:Domestic animal endocrinology 2000-11, Vol.19 (4), p.223-235
Main Authors: Blum, J.W, Dosogne, H, Hoeben, D, Vangroenweghe, F, Hammon, H.M, Bruckmaier, R.M, Burvenich, C
Format: Article
Language:English
Subjects:
Animals
Cattle
Cell Count - veterinary
Colony Count, Microbial
Endotoxins - pharmacology
Escherichia coli - pathogenicity
Escherichia coli Infections - blood
Escherichia coli Infections - pathology
Escherichia coli Infections - veterinary
Ethylenediamines
Female
Free Radical Scavengers
Leukocyte Count - veterinary
Mastitis, Bovine - blood
Mastitis, Bovine - pathology
Milk - chemistry
Nitric Oxide - biosynthesis
Nitrites - analysis
Nitrites - blood
Radioimmunoassay - veterinary
Sulfanilamides
Tumor Necrosis Factor-alpha - analysis
Tumor Necrosis Factor-alpha - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Concentrations of tumor necrosis factor-α (TNF-α) and of NO x (sum of nitrite and nitrate as indicators of endogenous nitric oxide production) in milk and blood plasma were measured in three mastitis models in dairy cows in early lactation. Escherichia coli P4:037 bacteria or endotoxin 0111:B4 were administered into both left quarters of 12 and 6 cows, respectively. Six of the E. coli-infected cows were treated with a bactericidal antibiotic (Enrofloxacin; Bayer AG, Leverkusen, Germany) i.v. at 10 hr and subcutaneously (sc) at 30 hr after infection. NO x concentrations transiently increased maximally 10- to 11-fold in milk of E. coli-infected quarters with or without antibiotic treatment at 24 hr and after endotoxin administration. NO x concentrations did not change in milk of unchallenged quarters and in blood plasma. Increases of NO x were proceeded by a transient (96- to 149-fold) rise of milk TNF-α concentrations, which in endotoxin-administered quarters was maximal at 6 hr and in infected quarters without or with Enrofloxacin treatment at 10 and 14 hr. In blood plasma TNF-α concentrations only moderately increased to peaks in endotoxin-administered cows at 6 hr and in E. coli-infected cows at 14 hr postchallenge. In one severely sick, nontreated E. coli-infected cow milk, TNF-α response at 14 hr was excessive and followed by a spectacular rise of NO x concentration in milk between 48 and 72 hr. In conclusion, a possible clinical relevance of nitric oxide production associated with a rise of intramammary and systemic TNF-α during acute mastitis by E. coli infection and endotoxin in lactating dairy cows is indicated, but could not be inhibited by antibiotic treatment.
ISSN:0739-7240
1879-0054
DOI:10.1016/S0739-7240(00)00079-5