Role of DNA polymerase II in the tolerance of thymine dimers remaining unexcised in UV-irradiated Escherichia coli exposed to pre-UV nutritional stress

Nutritional stress applied prior to UV-irradiation to E. coli 15 555-7 reduced thymine dimer excision and inhibited post-UV incorporation of thymidine in polB + as well as in polB − cells. However, the pre-UV-stressed polB + cells were significantly more UV-resistant and after UV synthesized larger...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2001-12, Vol.65 (2), p.145-150
Main Authors: Sedliakova, Milena, Slezarikova, Viera, Masek, Frantisek, Vizvaryova, Miriam, Pirsel, Miroslav
Format: Article
Language:English
Subjects:
Culture Media
DNA Damage
DNA polymerase II
DNA Polymerase II - physiology
DNA repair
DNA, Bacterial - radiation effects
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli Proteins - genetics
Escherichia coli Proteins - physiology
Kinetics
Lesion tolerance
Pyrimidine Dimers - radiation effects
Thymine dimers
Ultraviolet Rays
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Summary:Nutritional stress applied prior to UV-irradiation to E. coli 15 555-7 reduced thymine dimer excision and inhibited post-UV incorporation of thymidine in polB + as well as in polB − cells. However, the pre-UV-stressed polB + cells were significantly more UV-resistant and after UV synthesized larger DNA molecules than the pre-UV-stressed polB − cells. The data suggest that DNA polymerase II is involved in the tolerance of unremoved thymine dimers.
ISSN:1011-1344
1873-2682
DOI:10.1016/S1011-1344(01)00256-1