Disjunction of Homologous Chromosomes in Meiosis I Depends on Proteolytic Cleavage of the Meiotic Cohesin Rec8 by Separin

It has been proposed but never proven that cohesion between sister chromatids distal to chiasmata is responsible for holding homologous chromosomes together while spindles attempt to pull them toward opposite poles during metaphase of meiosis I. Meanwhile, the mechanism by which disjunction of homol...

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Bibliographic Details
Published in:Cell 2000-10, Vol.103 (3), p.387-398
Main Authors: Buonomo, Sara B.C, Clyne, Rosemary K, Fuchs, Joerg, Loidl, Josef, Uhlmann, Frank, Nasmyth, Kim
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Centromere - metabolism
Chromosome Segregation
Chromosomes, Fungal - metabolism
cohesin
Endodeoxyribonucleases
Endopeptidases
Esterases - genetics
Esterases - physiology
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungal Proteins - physiology
Genes, Fungal - genetics
Genes, Fungal - physiology
In Situ Hybridization, Fluorescence
Ligases
Meiosis - genetics
Mitosis
Models, Biological
Mutation - genetics
Phosphoproteins
Protein Processing, Post-Translational
Rec8 protein
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Scc1 protein
Schizosaccharomyces pombe Proteins
Separase
separin
Sequence Homology, Nucleic Acid
sister chromatids
Ubiquitin-Protein Ligases
Yeasts - cytology
Yeasts - enzymology
Yeasts - genetics
Yeasts - metabolism
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Summary:It has been proposed but never proven that cohesion between sister chromatids distal to chiasmata is responsible for holding homologous chromosomes together while spindles attempt to pull them toward opposite poles during metaphase of meiosis I. Meanwhile, the mechanism by which disjunction of homologs is triggered at the onset of anaphase I has remained a complete mystery. In yeast, cohesion between sister chromatid arms during meiosis depends on a meiosis-specific cohesin subunit called Rec8, whose mitotic equivalent, Scc1, is cleaved at the metaphase to anaphase transition by an endopeptidase called separin. We show here that cleavage of Rec8 by separin at one of two different sites is necessary for the resolution of chiasmata and the disjunction of homologous chromosomes during meiosis.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)00131-8