Pathologic validation of a new method to quantify coronary calcific deposits in vivo using intravascular ultrasound

Current methods of calcium quantification by intravascular ultrasound (IVUS) measure the arc of calcium using the cross-sectional image at the lesion and at the reference site while neglecting calcium elsewhere. Calcium at these sites may not adequately represent the extent of total epicardial coron...

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Bibliographic Details
Published in:The American journal of cardiology 2000, Vol.85 (1), p.37-40
Main Authors: Scott, Douglas S, Arora, Umesh K, Farb, Andrew, Virmani, Renu, Weissman, Neil J
Format: Article
Language:eng
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Summary:Current methods of calcium quantification by intravascular ultrasound (IVUS) measure the arc of calcium using the cross-sectional image at the lesion and at the reference site while neglecting calcium elsewhere. Calcium at these sites may not adequately represent the extent of total epicardial coronary calcium. We devised a new method to quantify calcium as a percentage of the coronary luminal surface. This study examines whether this new method accurately reflects coronary calcium determined by histology. Seventeen postmortem coronary arteries were pressure-fixed and imaged by IVUS using a motorized pullback device. Total plaque-luminal circumferential length and calcified plaque-luminal circumferential length were measured from serial cross-sectional IVUS images every 1 mm. With use of Simpson’s method, the total plaque and calcified plaque surface area was then calculated. Histologic sections were stained with hematoxylin-eosin and Movat pentachrome at 3-mm intervals. Calcium was independently quantified by planimetry under light microscopy. Histologic analysis (n = 253 sections) revealed a wide range of calcium (0 to 47 mm 2; mean 12 ± 16 mm 3). The IVUS-derived calcified plaque surface area was 17 ± 23 mm 2), which represented 3.1 ± 4.1% (range 0% to 13.9%) of the total plaque surface area. The histologic and IVUS quantification of calcium by this method was strongly related (r = 0.84, p
ISSN:0002-9149
1879-1913