Differentiation of Herpes Simplex Virus Types 1 and 2 in Sera of Patients with HSV Central Nervous System Infections by Type-specific Enzyme-linked Immunosorbent Assay

Objective: To differentiate by enzyme-linked immunosorbent assay (ELISA) using type-specific glycoprotein G herpes simplex virus (HSV) type 1 and type 2 in serum collected from patients with HSV central nervous system (CNS)infections. Methods: HSV 1 and 2 typing in convalescent sera of 17 patients w...

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Bibliographic Details
Published in:The Journal of infection 2001-10, Vol.43 (3), p.206-209
Main Authors: Nishimura, Y., Ayabe, M., Shoji, H., Hashiguchi, H., Eizuru, Y., Kawana, T.
Format: Article
Language:English
Subjects:
Adult
Aged
Antibodies, Viral - blood
Antibodies, Viral - cerebrospinal fluid
Biological and medical sciences
Central Nervous System Infections - virology
Enzyme-Linked Immunosorbent Assay
glycoprotein G
Herpes Simplex - virology
Herpes simplex virus 1
Herpes simplex virus 2
Herpesvirus 1, Human - isolation & purification
Herpesvirus 2, Human - isolation & purification
Human viral diseases
Humans
Infectious diseases
Male
Medical sciences
Middle Aged
Serologic Tests
Viral diseases
Viral diseases of the nervous system
Viral Envelope Proteins - immunology
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Summary:Objective: To differentiate by enzyme-linked immunosorbent assay (ELISA) using type-specific glycoprotein G herpes simplex virus (HSV) type 1 and type 2 in serum collected from patients with HSV central nervous system (CNS)infections. Methods: HSV 1 and 2 typing in convalescent sera of 17 patients with HSV acute encephalitis, myelitis, or meningitis was determined by the type-specific IgG ELISA kit (Gull Laboratory, Inc.). HSV CNS infections were diagnosed by polymerase chain reaction (PCR) or conventional serologic tests from acute to convalescent stages. Results: In 13 of 17 patients, HSV type 1 and HSV type 2 antibodies were positive; 11 patients with HSV type 1 and 2 patients with HSV type 2 were found. All 10 patients with encephalitis showed equivocal or positive results for HSVtype 1. In two of three cases of myelitis, HSV type 1 was demonstrated. Each case of myelitis and meningitis reacted to both types 1 and 2. Conclusion: These data suggest that the kit is useful for type differentiation of HSV CNS infections from convalescent sera, and can play a supplementary role in HSV typing by PCR or previous serologic tests.
ISSN:0163-4453
1532-2742
DOI:10.1053/jinf.2001.0881