Complementary DNA structure and genomic organization of Drosophila menin

Menin is a protein product of a tumor supressor gene MEN1, mutations of which are responsible for multiple endocrine neoplasia type 1, an autosomal dominant familial cancer syndrome. We determined the nucleotide sequence of the Drosophila menin cDNA using RT-PCR and RACE, and confirmed it by direct...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2000-10, Vol.168 (1), p.135-140
Main Authors: Maruyama, Kouji, Tsukada, Toshihiko, Honda, Munehiro, Nara-Ashizawa, Noriko, Noguchi, Kiyoteru, Cheng, Jinyan, Ohkura, Naganari, Sasaki, Kazuki, Yamaguchi, Ken
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Blotting, Northern
cDNA
DNA, Complementary
Drosophila
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Embryo, Nonmammalian
Exon/intron boundary
Exons
Genes, Insect
Genes, Tumor Suppressor - genetics
Homology
Humans
Insect Proteins - chemistry
Insect Proteins - genetics
Introns
MEN1
menin
Menin ( Drosophila)
Molecular Sequence Data
Multiple Endocrine Neoplasia Type 1 - genetics
Neoplasm Proteins - chemistry
Neoplasm Proteins - genetics
Northern blotting
Protein Structure, Tertiary
Proto-Oncogene Proteins
RNA, Messenger - metabolism
Sequence Alignment
Sequence Homology
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Summary:Menin is a protein product of a tumor supressor gene MEN1, mutations of which are responsible for multiple endocrine neoplasia type 1, an autosomal dominant familial cancer syndrome. We determined the nucleotide sequence of the Drosophila menin cDNA using RT-PCR and RACE, and confirmed it by direct sequencing of genomic DNA. Gene expression of Drosophila menin was detected by Northern blot analysis in adult and embryo as two types of transcripts, one identical in size to the cDNA, and the other larger but detected only in embryo. The Drosophila menin gene was composed of five exons in which the protein was encoded in exon 2 through 5, and spanned approximately 6.3 kb. The deduced amino acid (AA) sequence of Drosophila menin consisted of 751 AAs with a calculated molecular mass of 81.7 kDa, and showed 44–47% identity to human, rat, mouse and zebrafish menin over the entire length. Among the AA residue substitutions that have been reported as disease-associated missense mutations and single AA deletions, 53 out of 71 were completely conserved in Drosophila. The presence of menin ortholog in insect indicates that menin is an evolutionally conserved protein with a fundamental role in biological processes.
ISSN:0303-7207
1872-8057
DOI:10.1016/S0303-7207(00)00307-5