Increased oxidative damage to DNA in a transgenic mouse model of Huntington's disease

Mitochondrial dysfunction and oxidative damage may play a role in the pathogenesis of Huntington's disease (HD). We examined concentrations of 8‐hydroxy‐2‐deoxyguanosine (OH8dG), a well‐established marker of oxidative damage to DNA, in a transgenic mouse model of HD (R6/2). Increased concentrat...

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Bibliographic Details
Published in:Journal of neurochemistry 2001-12, Vol.79 (6), p.1246-1249
Main Authors: Bogdanov, Misha B., Andreassen, Ole A., Dedeoglu, Alpaslan, Ferrante, Robert J., Beal, M. Flint
Format: Article
Language:English
Subjects:
8‐hydroxy‐2‐deoxyguanosine
Animals
Biological and medical sciences
Biomarkers
Brain - metabolism
Cerebral Cortex - metabolism
Corpus Striatum - metabolism
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - analysis
DNA - metabolism
DNA Damage
Female
free radicals
Humans
Huntingtin Protein
Huntington Disease - genetics
Huntington Disease - metabolism
Huntington's disease
Male
Medical sciences
Mice
Mice, Transgenic
Microdialysis
Mitochondria - metabolism
Models, Animal
Nerve Degeneration
Nerve Tissue Proteins - genetics
Neurology
Nuclear Proteins - genetics
Oxidation-Reduction
oxidative damage
Oxidative Stress
transgenic
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Summary:Mitochondrial dysfunction and oxidative damage may play a role in the pathogenesis of Huntington's disease (HD). We examined concentrations of 8‐hydroxy‐2‐deoxyguanosine (OH8dG), a well‐established marker of oxidative damage to DNA, in a transgenic mouse model of HD (R6/2). Increased concentrations of OH8dG were found in the urine, plasma and striatal microdialysates of the HD mice. Increased concentrations were also observed in isolated brain DNA at 12 and 14 weeks of age. Immunocytochemistry showed increased OH8dG staining in late stages of the illness. These results suggest that oxidative damage may play a role in the pathogenesis of neuronal degeneration in the R6/2 transgenic mouse model of HD.
ISSN:0022-3042
1471-4159
DOI:10.1046/j.1471-4159.2001.00689.x