Understanding the Role of Stereoelectronic Effects in Determining Collagen Stability. 1. A Quantum Mechanical Study of Proline, Hydroxyproline, and Fluoroproline Dipeptide Analogues in Aqueous Solution

The importance of local (intraresidue) effects in determining the stability of the collagen triple helix has been investigated with special reference to the role played by hydroxyproline. To this end the dipeptide analogues of l-proline (ProDA), 4(R)-hydroxy-l-proline (HypDA), and 4(R)-fluoro-l-prol...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2001-12, Vol.123 (50), p.12568-12577
Main Authors: Improta, Roberto, Benzi, Caterina, Barone, Vincenzo
Format: Article
Language:English
Subjects:
Collagen - chemistry
Dipeptides - chemistry
Hydroxyproline - chemistry
Models, Molecular
Proline - analogs & derivatives
Proline - chemistry
Protein Conformation
Quantum Theory
Solutions
Stereoisomerism
Thermodynamics
Water - chemistry
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Summary:The importance of local (intraresidue) effects in determining the stability of the collagen triple helix has been investigated with special reference to the role played by hydroxyproline. To this end the dipeptide analogues of l-proline (ProDA), 4(R)-hydroxy-l-proline (HypDA), and 4(R)-fluoro-l-proline (FlpDA) have been studied by means of quantum mechanical ab initio calculations, taking into account solvent effects by the polarizable continuum model (PCM). Our results confirm that the relative stability of up puckerings of the pyrrolidine ring increases with the electronegativity of the 4(R) substituent (X), whereas down puckerings are favored by 4(S) electronegative substituents. Calculations on model compounds show that this effect is due to the interaction between vicinal C−H bonding and C−X antibonding orbitals. Electronegative substituents on the pyrrolidine ring affect cis−trans isomerism around the peptidic bond, with trans isomers stabilized by 4(R) substituents and cis isomers by 4(S) substituents. Also the hydrogen bonding power of the carbonyl moiety following the pyrrolidine ring is affected by 4(R) substituents, but this effect is tuned by the polarity of the embedding medium. Finally, up puckering favors smaller values of the backbone dihedrals φ and ψ. All these results strongly support the proposal that the stability of triple helices containing fluorinated or hydroxylated prolines in Y positions is related to the necessity of having up puckerings in those positions.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja010599i