Determination of Levamisole in Animal Tissues Using Liquid Chromatography with Ultraviolet Detection

An efficient and sensitive liquid chromatographic method is described for the determination of the anthelminthic drug levamisole, in muscle, liver, kidney and fat of sheep, pigs and poultry, using thiabendazole as internal standard. Samples were extracted by homogenizing with chloroform, and were ap...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2001-12, Vol.49 (12), p.5702-5705
Main Authors: Dreassi, Elena, Corbini, Gianfranco, La Rosa, Caterina, Politi, Norma, Corti, Piero
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chromatography, Ion Exchange
Food industries
Fundamental and applied biological sciences. Psychology
Levamisole - analysis
Levamisole - pharmacokinetics
Meat and meat product industries
Poultry
Reproducibility of Results
Sheep
Spectrophotometry, Ultraviolet
Swine
Tissue Distribution
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Summary:An efficient and sensitive liquid chromatographic method is described for the determination of the anthelminthic drug levamisole, in muscle, liver, kidney and fat of sheep, pigs and poultry, using thiabendazole as internal standard. Samples were extracted by homogenizing with chloroform, and were applied to Supelco Si solid-phase extraction columns and eluted with methanol. Chromatographic analysis was performed on a LiChrospher 60 RP−Select B column using methanol/ammonium acetate buffer 0.05 M (55/65, v/v) as mobile phase and reading at 220 nm. The quantification limit for the assay was 4 ng/g. Mean recoveries were about 84% for liver, 85% for kidney, 89% for muscle and 84% for fat. The assay has been used for statutory testing purposes. Keywords: Levamisole determination; anthelmintic; animal tissue; veterinary drug residues
ISSN:0021-8561
1520-5118
DOI:10.1021/jf0102362