Comparison of methods for erythroblast selection: Application to selecting fetal erythroblasts from maternal blood

Background Many methods have been employed to obtain fetal cells from maternal blood for prenatal diagnostics, but there has been little work done that compares the efficacy of different methods. This study presents a comparison of two commonly used methods for selecting erythroblasts with selection...

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Published in:Cytometry (New York, N.Y.) N.Y.), 2001-12, Vol.45 (4), p.267-276
Main Authors: Collarini, Ellen J., Cain, Carol A., Gammon, Dawn, Harriman, Bill, Magee, Kerry, Du, Gehua, Schueler, Paula A., Mahoney, Walt C.
Format: Article
Language:English
Subjects:
Acetazolamide - pharmacology
Centrifugation, Density Gradient - methods
erythroblasts
Erythroblasts - cytology
Erythroblasts - drug effects
Female
Fetal Blood - cytology
Fetal Blood - drug effects
fetal cells in maternal blood
Fetomaternal Transfusion
Gestational Age
Hemolysis
Humans
immunomagnetic selection
Immunomagnetic Separation - methods
In Vitro Techniques
Pregnancy - blood
prenatal diagnosis
Prenatal Diagnosis - methods
Receptors, Transferrin - immunology
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Summary:Background Many methods have been employed to obtain fetal cells from maternal blood for prenatal diagnostics, but there has been little work done that compares the efficacy of different methods. This study presents a comparison of two commonly used methods for selecting erythroblasts with selection directly from whole blood. Methods Erythroblasts were isolated from maternal blood by either differential lysis or density separation, followed by selection with an antibody to the transferrin receptor. These methods were compared with antibody selection directly from whole blood. The total yield of erythroblasts was determined for each method. Results Red cell lysis is not recommended because the lysis step cannot be well controlled. Density separation followed by antibody selection works well. However, a faster and simpler method, antibody selection directly from whole blood using Immunicon Ferrofluid and magnetic separators, works as well and has the potential to yield even more cells. Conclusions Considering the need for a simple and quick method for selecting fetal cells from maternal blood, we suggest selection directly from whole blood. Cytometry 45:267–276, 2001. © 2001 Wiley‐Liss, Inc.
ISSN:0196-4763
1097-0320
DOI:10.1002/1097-0320(20011201)45:4<267::AID-CYTO10023>3.0.CO;2-D