Bile Analysis as a Tool for Assessing Integrity of Biliary Epithelial Cells after Cold Ischemia–Reperfusion of Rat Livers

Previous morphological studies failed to show appreciable injury of biliary epithelial cells (BEC) after cold ischemia of rat liver, although recent evidence indicated that BEC integrity and function were impaired in this model. We tested the hypothesis that analysis of bile for enzymes, such as lac...

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Bibliographic Details
Published in:Cryobiology 2000-09, Vol.41 (2), p.145-152
Main Authors: Vajdová, Katarı́na, Smreková, Renáta, Kukan, Marián, Lutterová, Martina, Wsólová, Ladislava
Format: Article
Language:English
Subjects:
Alanine Transaminase - analysis
Animals
Aspartate Aminotransferases - analysis
Bile - enzymology
bile analysis
biliary epithelial cells
Biliary Tract - cytology
Biliary Tract - enzymology
Biological and medical sciences
Cold Temperature
Cryopreservation
Cytosol - enzymology
Epithelial Cells - cytology
Epithelial Cells - enzymology
gamma-Glutamyltransferase - analysis
Glucose - analysis
Hepatocytes - cytology
Hepatocytes - enzymology
Ischemia - enzymology
L-Lactate Dehydrogenase - analysis
lactate dehydrogenase
Liver - blood supply
Liver - enzymology
liver ischemia and reperfusion
Liver Transplantation
Liver, biliary tract, pancreas, portal circulation, spleen
Male
Medical sciences
Permeability
rat
Rats
Rats, Wistar
Reperfusion Injury - enzymology
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the digestive system
Temperature
Tight Junctions - ultrastructure
Time Factors
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Summary:Previous morphological studies failed to show appreciable injury of biliary epithelial cells (BEC) after cold ischemia of rat liver, although recent evidence indicated that BEC integrity and function were impaired in this model. We tested the hypothesis that analysis of bile for enzymes, such as lactate dehydrogenase (LDH), alanine transaminase (ALT), and aspartate transaminase (AST), can be used for assessing cold ischemic injury of BEC. Furthermore, we examined whether biliary γ-glutamyltransferase (GGT) reflects warm ischemic injury of BEC and whether normothermic reperfusion aggravates the negative effect of cold ischemia on BEC integrity and function. Rat livers were reperfused after different periods of cold or warm ischemia using a blood-free perfusion model. Compared with controls, perfusate LDH, ALT, and AST levels and parameters of hepatocyte function, including hepatocyte tight junction permeability, were not significantly altered by 18-h cold ischemia. On the other hand, 9-h cold ischemia markedly increased biliary LDH, ALT, and AST levels. However, only LDH release into the bile was strongly dependent on the time of cold storage. Biliary GGT, LDH, and glucose levels decreased during the reperfusion period following 18-h cold ischemia. The results suggest that biliary LDH can be used for assessing injury of BEC in cold-preserved livers and that normothermic reperfusion does not aggravate preservation-induced injury of BEC after cold ischemic storage.
ISSN:0011-2240
1090-2392
DOI:10.1006/cryo.2000.2276