Distinctive amygdala kindled seizures differentially affect neurobehavioral recovery and lesion-induced basic fibroblast growth factor (bFGF) expression

The differing effects of partial seizures on neurobehavioral recovery following anteromedial cortex (AMC) injury in rats have previously been reported. Specifically, convulsive Stage 1 seizures evoked ipsilateral to the lesion during the 6-day post-lesion critical period delayed recovery, while non-...

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Bibliographic Details
Published in:Brain research 2000-10, Vol.880 (1), p.38-50
Main Authors: Kline, Anthony E, Montañez, Sylvia, Bradley, Hallie A, Millar, Courtney J, Hernandez, Theresa D
Format: Article
Language:English
Subjects:
Amygdala - physiology
Amygdala - physiopathology
Analysis of Variance
Animals
Anteromedial cortex
Biological and medical sciences
c-Fos
Cerebral Cortex - physiology
Cerebral Cortex - physiopathology
Fibroblast Growth Factor 2 - genetics
Functional Laterality
Gene Expression Regulation - physiology
Genes, fos
Growth factor
Immunohistochemistry
Injuries of the nervous system and the skull. Diseases due to physical agents
Kindling, Neurologic - physiology
Male
Medical sciences
Proto-Oncogene Proteins c-fos - analysis
Proto-Oncogene Proteins c-fos - genetics
Rats
Rats, Long-Evans
Seizures - physiopathology
Time Factors
Trauma
Traumas. Diseases due to physical agents
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Summary:The differing effects of partial seizures on neurobehavioral recovery following anteromedial cortex (AMC) injury in rats have previously been reported. Specifically, convulsive Stage 1 seizures evoked ipsilateral to the lesion during the 6-day post-lesion critical period delayed recovery, while non-convulsive Stage 0 seizures were neutral. The present study was designed to elaborate on that research by examining several potential mechanisms for the seizure-associated difference observed in functional outcome. Anesthetized rats sustained unilateral AMC lesions followed by implantation of a stimulating electrode in the amygdala ipsilateral (Expt. 1) or contralateral (Expt. 2) to the lesion. Beginning 48 h after surgery, animals were kindled to evoke Stage 0 or Stage 1 seizure activity during the critical period. Kindling trials and afterdischarge (AD) were controlled to ascertain their role in functional outcome. Recovery from somatosensory deficits was assessed over a two-month period. The results revealed that (i) Stage 0 seizures did not impact recovery regardless of whether initiated ipsilateral or contralateral to the lesion, (ii) Stage 1 seizures prevented recovery only when initiated in the ipsilateral hemisphere during the post-lesion critical period, and (iii) the detrimental effect of Stage 1 seizures appears to be independent of the number of kindling trials provided and cumulative AD. Thus, to determine why Stage 1 seizures evoked in the hemisphere ipsilateral to the lesion impeded recovery, a separate group of animals (Expt. 3) were kindled accordingly and processed for c-Fos and basic fibroblast growth factor (bFGF) immunohistochemistry. It was hypothesized that Stage 1 seizures evoked in the injured hemisphere prevent recovery by blocking lesion-induced bFGF expression in structures shown to be important for recovery after cortex lesions (e.g., striatum). The results confirmed our hypothesis and suggest that the seizure-associated inhibition of lesion-induced bFGF may alter the growth factor-mediated plasticity necessary for functional recovery.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(00)02762-1