Antigen Presentation in Extracellular Matrix: Interactions of T Cells with Dendritic Cells Are Dynamic, Short Lived, and Sequential

Cognate interactions of naive T cells with antigen-presenting dendritic cells require physical cell–cell contacts leading to signal induction and T cell activation. Using a three-dimensional collagen matrix videomicroscopy model for ovalbumin peptide-specific activation of murine and oxidative mitog...

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Bibliographic Details
Published in:Immunity (Cambridge, Mass.) Mass.), 2000-09, Vol.13 (3), p.323-332
Main Authors: Gunzer, Matthias, Schäfer, Angelika, Borgmann, Stefan, Grabbe, Stephan, Zänker, Kurt S., Bröcker, Eva-Bettina, Kämpgen, Eckhart, Friedl, Peter
Format: Article
Language:English
Subjects:
Animals
Antigen Presentation - immunology
Antigen-Presenting Cells - cytology
Antigen-Presenting Cells - immunology
Antigen-Presenting Cells - metabolism
Bone Marrow Cells - cytology
Bone Marrow Cells - immunology
Bone Marrow Cells - metabolism
Calcium Signaling - immunology
Cell Adhesion - immunology
Cell Aggregation - immunology
Cell Communication - immunology
Cell Movement - immunology
Cells, Cultured
Collagen - immunology
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - metabolism
Extracellular Matrix - immunology
Extracellular Matrix - metabolism
Humans
Lymphocyte Activation - immunology
Mice
Mice, Inbred BALB C
Mice, Transgenic
Microscopy, Video
Oxidation-Reduction
T-Lymphocytes - cytology
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Time Factors
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Summary:Cognate interactions of naive T cells with antigen-presenting dendritic cells require physical cell–cell contacts leading to signal induction and T cell activation. Using a three-dimensional collagen matrix videomicroscopy model for ovalbumin peptide-specific activation of murine and oxidative mitogenesis of human T cells, we show that T cells maintain vigorous migration upon cognate interactions to DC (dendritic cell), continuously crawl across the DC surface, and rapidly detach (median within 6–12 min). These dynamic and short-lived encounters favor sequential contacts with the same or other DC and trigger calcium influx, upregulation of activation markers, T blast formation, and proliferation. We conclude that a tissue environment supports the accumulation of sequential signals, implicating a numeric or “digital” control mechanism for an ongoing primary immune response.
ISSN:1074-7613
1097-4180
DOI:10.1016/S1074-7613(00)00032-7