Candidate Neisseria meningitidis NspA vaccine

The highly conserved NspA protein has been found in the outer membrane of every Neisseria meningitidis strain tested so far. Two monoclonal antibodies (MAbs) directed against this protein were used to demonstrate that biologically important epitopes of the NspA protein are exposed at the surface of...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biotechnology 2000-09, Vol.83 (1), p.27-31
Main Authors: Martin, Denis, Brodeur, Bernard R, Hamel, Josée, Couture, France, de Alwis, Uditha, Lian, Zhirui, Martin, Susan, Andrews, David, Ellis, Ronald W
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Monoclonal - immunology
Bacterial Outer Membrane Proteins - chemistry
Bacterial Outer Membrane Proteins - immunology
Bacterial Vaccines - immunology
Biotechnology
Cell membranes
Cross Reactions
Diseases
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Immunization
Immunology
Living systems studies
Meningococcal vaccine
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Monoclonal antibodies
Neisseria meningitidis
Neisseria meningitidis - immunology
NspA protein
Outer membrane protein
outer membrane proteins
Proteins
Recombinant Proteins - chemistry
Recombinant Proteins - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The highly conserved NspA protein has been found in the outer membrane of every Neisseria meningitidis strain tested so far. Two monoclonal antibodies (MAbs) directed against this protein were used to demonstrate that biologically important epitopes of the NspA protein are exposed at the surface of serologically distinct meningococcal strains. Analysis of sera collected from mice that survived a deadly meningococcal challenge following immunization with recombinant NspA protein (rNspA) revealed the presence of cross-reactive antibodies which efficiently attached to and killed the four serogroup B strains tested. These data are additional proof that the NspA protein is exposed at the surface of intact meningococcal cells, which is an important characteristic for a vaccine candidate.
ISSN:0168-1656
1873-4863
DOI:10.1016/S0168-1656(00)00294-7