Is Collagen Breakdown During Periodontitis Linked to Inflammatory Cells and Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Human Gingival Tissue?

Background: Evidence of the role of matrix metalloproteinases (MMPs) produced by resident and inflammatory cells in periodontal destruction is now well established. The purpose of this study was to quantify, in healthy and diseased upper gingival connective tissue, the area fraction (AA%) occupied b...

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Bibliographic Details
Published in:Journal of periodontology (1970) 2001-10, Vol.72 (10), p.1398-1406
Main Authors: Séguier, Sylvie, Gogly, Bruno, Bodineau, Agnès, Godeau, Gaston, Brousse, Nicole
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Antigens, CD - analysis
Antigens, Differentiation, B-Lymphocyte - analysis
Antigens, Differentiation, Myelomonocytic - analysis
Azo Compounds
B-Lymphocytes - pathology
Blotting, Western
CD4-Positive T-Lymphocytes - pathology
CD8-Positive T-Lymphocytes - pathology
Cell Adhesion Molecules
Cells, inflammatory
collagen
Collagen - metabolism
Coloring Agents
comparison studies
Culture Techniques
Dentistry
Female
Gingiva - enzymology
Granulocyte Colony-Stimulating Factor - analysis
Humans
Image Processing, Computer-Assisted
Immunoblotting
Lectins
Leukocytes, Mononuclear - metabolism
Leukocytes, Mononuclear - pathology
Macrophages - pathology
Male
matrix metalloproteinase tissue inhibitors
matrix metalloproteinases
Matrix Metalloproteinases - analysis
Matrix Metalloproteinases - metabolism
Membrane Proteins - analysis
Middle Aged
Monocytes - pathology
periodontal diseases/pathology
Periodontitis - enzymology
Periodontitis - metabolism
Periodontitis - pathology
Phagocytes - metabolism
Phagocytes - pathology
Poly(A)-Binding Proteins
Proteins
RNA-Binding Proteins - analysis
Sialic Acid Binding Ig-like Lectin 2
Statistics as Topic
T-Cell Intracellular Antigen-1
Tissue Inhibitor of Metalloproteinases - analysis
Tissue Inhibitor of Metalloproteinases - metabolism
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Summary:Background: Evidence of the role of matrix metalloproteinases (MMPs) produced by resident and inflammatory cells in periodontal destruction is now well established. The purpose of this study was to quantify, in healthy and diseased upper gingival connective tissue, the area fraction (AA%) occupied by collagen fibers, the cell number belonging to inflammatory cell subsets, and the amounts of MMPs and TIMPs (tissue inhibitors of MMPs) in order to investigate the possible correlations, if any, between such molecules, collagen loss, and inflammatory cell subsets. Methods: Gingival tissue specimens from 6 healthy controls (C) and 6 patients with severe periodontitis (P) were divided into 2 groups. The first group of specimens was frozen and used for the staining of collagen fibers by sirius red F3Ba and for immunohistochemistry with antibodies against CD8, CD4, CD22, CD68, and TIA‐1 molecules. The second group was used for organ culture, zymography, Western blotting, and dot blotting. Morphometric and automated image analysis was performed for the evaluation of the area fraction occupied by collagen fibers, the number of inflammatory cell subsets and for enzymatic activities developed by MMPs, and the amounts of TIMPs expressed during periodontal disease. Results: In group P, the area fraction of collagen fibers (33 ± 10%) was significantly decreased (P
ISSN:0022-3492
1943-3670
DOI:10.1902/jop.2001.72.10.1398