Sensitive high-performance thin-layer chromatographic method for the estimation of diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana Roxb

Diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana was isolated and characterised. A sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the estimation of diospyrin. The method was validated for precision (intra- and inter-day), repeatabil...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2000-07, Vol.744 (2), p.257-262
Main Authors: Ravishankara, M.N, Shrivastava, Neeta, Jayathirtha, M.G, Padh, Harish, Rajani, M
Format: Article
Language:English
Subjects:
Antineoplastic Agents, Phytogenic - analysis
Antineoplastic drugs. Cytokines
Biological and medical sciences
Biotechnology
Chromatography, Thin Layer - methods
Diospyrin
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Magnoliopsida - chemistry
Naphthoquinones - analysis
Plant Stems - chemistry
Production of active biomolecules
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
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Summary:Diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana was isolated and characterised. A sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the estimation of diospyrin. The method was validated for precision (intra- and inter-day), repeatability and accuracy. The method was found to be precise, with the RSDs for intra-day in the range of 0.72–1.85% and RSDs for inter-day in the range of 1.06–2.95%, for different concentrations. Instrumental precision and repeatability of the method were found to be 0.086 and 0.937 (% CV), respectively. Accuracy of the method was checked by performing the recovery study at two levels and average percentage recovery was found to be 97.87%. The developed HPTLC method was adopted for the estimation of diospyrin content of the stem bark of D. montana from different regions, which varied from 0.35 to 0.47% (w/w) in the samples.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00277-2