Evaluation of a biochemical test scheme for identifying clinical isolates of Enterococcus faecalis and Enterococcus faecium

Aims: To evaluate the full test scheme of Facklam and Sahm (1995) for the identification of clinical enterococcal isolates to genus and species level. Methods and Results: Fifty‐nine clinical isolates, previously provisionally classed as enterococci on the basis of just four biochemical tests of Fac...

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Bibliographic Details
Published in:Letters in applied microbiology 2001-11, Vol.33 (5), p.392-396
Main Authors: Day, A.M., Sandoe, J.A.T., Cove, J.H., Phillips‐Jones, M.K.
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques - methods
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Enterococcus faecalis
Enterococcus faecalis - classification
Enterococcus faecalis - growth & development
Enterococcus faecalis - isolation & purification
Enterococcus faecalis - metabolism
Enterococcus faecium
Enterococcus faecium - classification
Enterococcus faecium - growth & development
Enterococcus faecium - isolation & purification
Enterococcus faecium - metabolism
Fundamental and applied biological sciences. Psychology
Gram-Positive Bacterial Infections - microbiology
Humans
Microbiology
Reproducibility of Results
Species Specificity
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Summary:Aims: To evaluate the full test scheme of Facklam and Sahm (1995) for the identification of clinical enterococcal isolates to genus and species level. Methods and Results: Fifty‐nine clinical isolates, previously provisionally classed as enterococci on the basis of just four biochemical tests of Facklam and Sahm and one other test, were subjected to genus and species identification using the full identification scheme of Facklam and Sahm; 98% of these strains were confirmed to be enterococci and of these, 69% were identified as Enterococcus faecalis and 31% as Enterococcus faecium. Six tests in the scheme (out of 24) gave anomalous or unreliable results for some strains, and two gave unexpected results for the majority of strains presumptively identified as Ent. faecium. Conclusions: Nine (out of 12) genus tests and nine (out of 12) species tests from the Facklam and Sahm scheme were reliable. Testing for the presence of the Lancefield antigen D was also useful. The majority of presumptive Ent. faecium strains gave different results for the sorbitol and raffinose tests from that expected. Significance and Impact of the Study: This study indicates the level of reliability for each of the tests in a current enterococcal identification scheme for differentiating clinical isolates, and showed that two tests gave consistently different test results from those expected for Ent. faecium.
ISSN:0266-8254
1472-765X
1365-2673
DOI:10.1046/j.1472-765X.2001.01017.x