Cloning, Characterization, and Phylogenetic Analysis of a Shrimp White Spot Syndrome Virus Gene That Encodes a Protein Kinase

An open reading frame (ORF) that encodes a 715-amino-acid polypeptide was found in an 8421-bp EcoRI fragment of the shrimp white spot syndrome virus (WSSV) genome. The polypeptide shows significant homology to eukaryotic serine/threonine protein kinase (PK) and contains the major conserved subdomain...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2001-10, Vol.289 (2), p.362-377
Main Authors: Liu, Wang-Jing, Yu, Hon-Tsen, Peng, Shao-En, Chang, Yun-Shiang, Pien, Hsiu-Wen, Lin, Ching-Ju, Huang, Chiu-Jung, Tsai, Meng-Feng, Huang, Chang-Jen, Wang, Chung-Hsiung, Lin, Jung-Yaw, Lo, Chu-Fang, Kou, Guang-Hsiung
Format: Article
Language:English
Subjects:
3' Untranslated Regions - genetics
5' Untranslated Regions - genetics
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Decapoda (Crustacea) - virology
DNA Viruses - classification
DNA Viruses - enzymology
DNA Viruses - genetics
double prime pk gene
Genes, Viral
Marine
Molecular Sequence Data
Molecular Weight
Natantia
Open Reading Frames
Penaeus monodon
Phylogeny
protein kinase
Protein Kinases - chemistry
Protein Kinases - classification
Protein Kinases - genetics
RNA, Messenger - analysis
RNA, Viral - analysis
Taiwan
White spot syndrome virus
WSSV pk gene
WSSV Taiwan isolate
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Description
Summary:An open reading frame (ORF) that encodes a 715-amino-acid polypeptide was found in an 8421-bp EcoRI fragment of the shrimp white spot syndrome virus (WSSV) genome. The polypeptide shows significant homology to eukaryotic serine/threonine protein kinase (PK) and contains the major conserved subdomains for eukaryotic protein kinases. Coupled in vitro transcription and translation generated a protein having an apparent molecular mass of about 87 kDa according to sodium dodecyl sulfate–polyacrylamide gel electrophoresis. For transcriptional analysis of the pk gene, total RNA was isolated from WSSV-infected shrimp at different times after infection. Northern blot analysis with pk-specific riboprobe found a major and a minor transcript of 2.7 and 5.7 kb, respectively. Rapid amplification of the 5′ cDNA ends of the major 2.7-kb pk transcript showed that there were two transcriptional initiation sites located at nucleotide residues -38(G) and -39(G) relative to the ATG translational start codon. Temporal expression analysis by RT-PCR indicated that the transcription of the pk gene started 2 h after infection and continued for at least 60 h. Phylogenetic analysis showed that WSSV protein kinase does not have any close relatives and does not fall into any of the major protein kinase groups.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2001.1091