High-Dose Methylprednisolone Reduces Cytokine-Induced Adhesion Molecules on Human Brain Endothelium

We investigated the in vitro effects of low- and high-dose methylprednisolone (MP) on the cytokine-induced expression of HLA-DR, ICAM-1 and VCAM-1 on human brain microvessel endothelial cells (HBMECs). Brain endothelium was obtained from microvessels included in the apparently normal white matter of...

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Bibliographic Details
Published in:Canadian journal of neurological sciences 2000-08, Vol.27 (3), p.241-244
Main Authors: Gelati, Maurizio, Corsini, Elena, Dufour, Anna, Massa, Giorgio, Giombini, Sergio, L.Solero, Carlo, Salmaggi, Andrea
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Cell Adhesion Molecules - antagonists & inhibitors
Cell Adhesion Molecules - metabolism
Cerebrovascular Circulation - drug effects
Dose-Response Relationship, Drug
Endothelium, Vascular - drug effects
Endothelium, Vascular - metabolism
Female
Glucocorticoids - administration & dosage
Glucocorticoids - pharmacology
HLA-DR Antigens - metabolism
Hormones. Endocrine system
Humans
Intercellular Adhesion Molecule-1 - metabolism
Interferon-gamma - pharmacology
Male
Medical sciences
Methylprednisolone - administration & dosage
Methylprednisolone - pharmacology
Middle Aged
Original Articles
Pharmacology. Drug treatments
Tumor Necrosis Factor-alpha - pharmacology
Vascular Cell Adhesion Molecule-1 - metabolism
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Summary:We investigated the in vitro effects of low- and high-dose methylprednisolone (MP) on the cytokine-induced expression of HLA-DR, ICAM-1 and VCAM-1 on human brain microvessel endothelial cells (HBMECs). Brain endothelium was obtained from microvessels included in the apparently normal white matter of surgical specimens of nine patients. Cells were stained with monoclonal antibodies anti-HLA-DR, anti-ICAM-1 and anti-VCAM-1 and analysed by flow cytometry as fluorescence histograms. The mean fluorescence intensity (MFI) of HBMECs treated with different stimuli was calculated. gamma-IFN-induced HLA-DR was down-regulated in a dose-dependent manner by MP. High-dose MP reduced the TNF-alpha-induced ICAM-1 and VCAM-1 expression. The down-regulation of adhesion molecules on cerebral endothelial cells could decrease mononuclear cell transmigration through the blood brain barrier and consequently the perivascular infiltrates. The results add support to the rationale for high-dose MP treatment in multiple sclerosis relapses.
ISSN:0317-1671
2057-0155
DOI:10.1017/S0317167100000883