Telomerase activity, and tissue polypeptide specific antigen (TPS) in Egyptian breast cancer patients

Objectives: Breast cancer is the most common malignancy among Egyptian women. The aim of this study is to evaluate the role of both telomerase and TPS estimation in assessment of breast cancer. Methods: The study included 40 patients with breast cancer, and 20 patients with benign breast diseases. T...

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Bibliographic Details
Published in:Clinical biochemistry 2001-09, Vol.34 (6), p.499-504
Main Authors: Kassim, Samar K, Fawzy, Hala, El Rassad, Moustafa M, Khalil, Fawzeya K, Khalifa, Ali
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biomarkers - analysis
Breast Diseases - diagnosis
Breast Diseases - metabolism
Breast Neoplasms - chemistry
Breast Neoplasms - diagnosis
Breast Neoplasms - metabolism
Carcinoma - chemistry
Carcinoma - diagnosis
Carcinoma - metabolism
Diagnosis, Differential
Disease Progression
Egypt
Enzyme-Linked Immunosorbent Assay
Female
Follow-Up Studies
Humans
Invasive duct carcinoma
Middle Aged
Neoplasm Staging
Neoplasms - chemistry
Neoplasms - diagnosis
Neoplasms - metabolism
Non-invasive duct carcinoma
Peptides - blood
Polymerase Chain Reaction
Predictive Value of Tests
Prognosis
ROC Curve
Sensitivity and Specificity
Telomerase
Telomerase - analysis
Telomerase - metabolism
Telomeric repeat amplification protocol
Tissue polypeptide specific antigen
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Summary:Objectives: Breast cancer is the most common malignancy among Egyptian women. The aim of this study is to evaluate the role of both telomerase and TPS estimation in assessment of breast cancer. Methods: The study included 40 patients with breast cancer, and 20 patients with benign breast diseases. Telomerase activity in breast tissues was assessed using TRAP assay. TPS was measured in sera of the patients by ELISA. Results: Telomerase positivity was 15% in benign group vs. 60% in malignant group ( p = 0.0009). It was significantly correlated to stage, and lymph node status ( p < 0.02). Telomerase positivity showed significant correlation to tumor recurrence ( p = 0.0076) in a follow-up period of 36 months. Mean rank of TPS was significantly higher in malignant than benign groups ( p < 0.001), and in telomerase positive than telomerase negative patients ( p < 0.001). In malignant group, mean rank of TPS was significantly higher in late stages ( p < 0.002), in higher grade ( p < 0.05), in larger tumor size ( p < 0.01), and in lymph node positive patients ( p < 0.001). ROC curve was utilized to choose the best cutoff for serum TPS (88 U/L). At this cutoff, the sensitivity was 95%, and the specificity was 75%. At a higher cutoff (109 U/L), TPS positivity was significantly correlated to stage, grade, lymph node status, and telomerase positivity ( p < 0.05). Conclusion: Telomerase positivity and serum TPS might be used as additional markers for assessment of breast cancer.
ISSN:0009-9120
1873-2933
DOI:10.1016/S0009-9120(01)00253-3