Vitamin A deficiency causes oxidative damage to liver mitochondria in rats

Mitochondrial damage in rat liver induced by chronic vitamin A-deficiency was studied using three different groups of rats: (i) control rats, (ii) rats fed a vitamin A-free diet until 50 d after birth and (iii) vitamin A-deficient rats re-fed a control diet for 30 d. No statistical difference in bod...

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Published in:Free radical biology & medicine 2000-07, Vol.29 (1), p.1-7
Main Authors: Barber, Teresa, Borrás, Elisa, Torres, Luis, Garcı́a, Concha, Cabezuelo, Francisco, Lloret, Ana, Pallardó, Federico V, Viña, Juan R
Format: Article
Language:English
Subjects:
Aging
Animals
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - metabolism
Energy Intake
Female
Flow Cytometry
Free radicals
Glutathione - metabolism
Glutathione Disulfide - metabolism
GSH
Intracellular Membranes - drug effects
Intracellular Membranes - physiology
Liver
Malondialdehyde - metabolism
Membrane Lipids - chemistry
Membrane Potentials - drug effects
Mitochondria
Mitochondria, Liver - drug effects
Mitochondria, Liver - metabolism
Mitochondria, Liver - physiology
mtDNA
Oxidative stress
Rats
Vitamin A
Vitamin A - blood
Vitamin A - pharmacology
Vitamin A Deficiency - metabolism
Vitamin A Deficiency - physiopathology
Weight Gain
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Summary:Mitochondrial damage in rat liver induced by chronic vitamin A-deficiency was studied using three different groups of rats: (i) control rats, (ii) rats fed a vitamin A-free diet until 50 d after birth and (iii) vitamin A-deficient rats re-fed a control diet for 30 d. No statistical difference in body weight and food intake was found between control and vitamin A-deficient rats. Liver GSH concentration was similar in both groups. However, in vitamin A-deficient rats, the mitochondrial GSH/GSSG ratio was significantly lower and the levels of malondialdehyde (MDA) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (oxo8dG) were higher when compared to control rats. These values were partially restored in re-fed rats. The mitochondrial membrane potential of vitamin A-deficient rats was significantly lower than in control rats and returned to normal levels in restored vitamin A rats. Two populations of mitochondria were found in vitamin A-deficient rats according to the composition of membrane lipids. One population showed a similar pattern to the control mitochondria and the second population had a higher membrane lipid content. This report emphasizes the protective role of vitamin A in liver mitochondria under physiological circumstances.
ISSN:0891-5849
1873-4596
DOI:10.1016/S0891-5849(00)00283-5