Induction of vitellogenin gene transcription in vitro by juvenile hormone in Blattella germanica

In the cockroach Blattella germanica, the synthesis of vitellogenin is juvenile hormone III (JH III)-dependent. We have studied the effect of JH III upon vitellogenin gene expression in periovaric fat bodies incubated in vitro. Periovaric fat bodies were obtained from cardioallatectomized females. T...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2001-10, Vol.183 (1), p.93-100
Main Authors: Comas, Daniel, Piulachs, Maria-Dolors, Bellés, Xavier
Format: Article
Language:English
Subjects:
Animals
Blattella germanica
Blattellidae
Blattellidae - genetics
Blattellidae - metabolism
Brain Chemistry
Corpora Allata - chemistry
Corpora Allata - physiology
Cycloheximide
Cycloheximide - pharmacology
Fat Body - chemistry
Fat Body - drug effects
Fat Body - physiology
Female
Gene Expression Regulation
German cockroach
In Vitro Techniques
Insect Hormones - pharmacology
Juvenile hormone
Neuropeptides - pharmacology
Neurosecretory Systems - chemistry
Neurosecretory Systems - physiology
Protein Synthesis Inhibitors - pharmacology
Reproduction
Sesquiterpenes - pharmacology
Vitellogenin
vitellogenin gene
Vitellogenins - genetics
Vitellogenins - metabolism
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Summary:In the cockroach Blattella germanica, the synthesis of vitellogenin is juvenile hormone III (JH III)-dependent. We have studied the effect of JH III upon vitellogenin gene expression in periovaric fat bodies incubated in vitro. Periovaric fat bodies were obtained from cardioallatectomized females. The response to JH III was measured in terms of vitellogenin and vitellogenin mRNA after 7 h of incubation. A hormonal concentration as low as 1 nM was enough to induce vitellogenin production and its release to the medium, whereas the concentration of 10 nM produced the maximal effects. Although the response of the vitellogenin gene to JH III is fast and efficient, it seems that the action is mediated by protein factors, given that cycloheximide treatment impairs the hormonal effect. The presence in the medium of brain extract (0.5 equivalents), corpora cardiaca (one pair) or hypertrehalosemic hormone (10 −7 or 10 −8 M), partially inhibited the response to JH III.
ISSN:0303-7207
1872-8057
DOI:10.1016/S0303-7207(01)00589-5