Immunohistochemistry on Cell Blocks From Fine-Needle Cytopunctures of Primary Breast Carcinomas and Lymph Node Metastases

We assessed the reliability of prognostic biologic markers by means of immunohistochemistry on cell blocks obtained from diagnostic fine-needle cytopunctures of breast carcinomas and their lymph node metastases. Immunohistochemical studies of MIB-1 (Ki-67), estrogen receptors (ER), progesterone rece...

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Bibliographic Details
Published in:Modern pathology 2000-08, Vol.13 (8), p.841-850
Main Authors: Briffod, Marianne, Hacène, Kamel, Le Doussal, Viviane
Format: Article
Language:English
Subjects:
Biomarkers, Tumor - analysis
Biopsy, Needle
Breast cancer
Breast Neoplasms - chemistry
Breast Neoplasms - pathology
Carcinoma in Situ - chemistry
Carcinoma in Situ - secondary
Carcinoma, Ductal, Breast - chemistry
Carcinoma, Ductal, Breast - secondary
Cell block
Cell Count
Female
Fine-needle cytopuncture
Humans
Immunohistochemistry
Lymph node metastasis
Lymph Nodes - pathology
Lymphatic Metastasis - pathology
Neoplasm Proteins - analysis
Receptors, Estrogen - analysis
Receptors, Progesterone - analysis
Reproducibility of Results
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Summary:We assessed the reliability of prognostic biologic markers by means of immunohistochemistry on cell blocks obtained from diagnostic fine-needle cytopunctures of breast carcinomas and their lymph node metastases. Immunohistochemical studies of MIB-1 (Ki-67), estrogen receptors (ER), progesterone receptors (PR), p53, and c-erb-B-2 were performed in 55 cases of primary breast carcinoma on cell blocks (cytoblock technique) and on their corresponding tissue samples (46 mastectomy specimens and 9 Trucut biopsies) and in 38 cases on cell blocks from fine-needle cytopunctures of both the primary breast tumors and their concurrent lymph node metastases. Interobserver reproducibility ranged from 87 to 100%, depending on the marker. A good correlation was observed between immunostaining assessment on cell blocks and on the corresponding tumor tissues as follows: Ki-67 (85%), ER (96%), PR (82%), p53 (76%), and c-erb-B-2 (84%). An excellent correlation was observed between cell-block results for primary tumors and node metastases; however, a far higher percentage of Ki-67–positive nuclei was observed in the nodes than in the corresponding tumors in seven cases. All nodes corresponding to ER- or PR-negative tumors were also negative, whereas the nodes corresponding to two ER-positive and one PR-positive tumor were negative. Marked discrepancies were also noted with p53 in two cases and with c-erb-B-2 in two cases. Most discrepancies occurred with Trucut biopsies and with breast tumors that contained a large intraductal component. We conclude that cell blocks prepared from fine-needle cytopuncture specimens of breast carcinomas and their node metastases are useful when planning neoadjuvant treatment.
ISSN:0893-3952
1530-0285
DOI:10.1038/modpathol.3880149