Neutralization of Human Papillomavirus Type 11 (HPV-11) by Serum from Women Vaccinated with Yeast-Derived HPV-11 L1 Virus-like Particles: Correlation with Competitive Radioimmunoassay Titer

Neutralization of human papillomavirus type 11 (HPV-11) has been demonstrated using serum and cervical secretions from primates vaccinated with virus-like particles (VLPs). Theoretically, neutralizing antibodies could protect women from HPV infection. The immunogenicity of a yeast-derived HPV-11 L1...

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Bibliographic Details
Published in:The Journal of infectious diseases 2001-11, Vol.184 (9), p.1183-1186
Main Authors: Brown, Darron R., Bryan, Janine T., Schroeder, Jill M., Robinson, Tiffany S., Fife, Kenneth H., Wheeler, Cosette M., Barr, Eliav, Smith, Paula R., Chiacchierini, Lisa, DiCello, Anthony, Jansen, Kathrin U.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animals
Antibodies, Viral - blood
Antibodies, Viral - immunology
Biological and medical sciences
Dose-Response Relationship, Drug
Double-Blind Method
Female
Human papillomavirus 11
Human viral diseases
Humans
Infectious diseases
Medical sciences
Mice
Neutralization Tests
Papillomaviridae - genetics
Papillomaviridae - immunology
Papillomavirus Infections - prevention & control
Papillomavirus Infections - virology
Radioimmunoassay
Tumor Virus Infections - prevention & control
Tumor Virus Infections - virology
Vaccination
Vaccines, Synthetic - immunology
Viral diseases
Viral diseases of the genital and urinary system
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Vaccines - immunology
Virion - immunology
Yeasts - genetics
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Summary:Neutralization of human papillomavirus type 11 (HPV-11) has been demonstrated using serum and cervical secretions from primates vaccinated with virus-like particles (VLPs). Theoretically, neutralizing antibodies could protect women from HPV infection. The immunogenicity of a yeast-derived HPV-11 L1 VLP vaccine was tested in women. Serum specimens were evaluated for HPV-11 titer by competitive radioimmunoassay (cRIA) and for neutralization by use of the athymic mouse xenograft system. Analysis of serum from 104 subjects showed a dose response in HPV-11 cRIA titers and neutralization. Overall, 68 (82.9%) of 82 postimmunization serum specimens from VLP recipients were 100% neutralizing when used in the assay at a 1:50 dilution. Of 69 serum specimens, 63 (91.3%) with cRIA titers >200 milliMerck units per milliliter were neutralizing. Immunization with HPV VLPs elicits a vigorous serum immune response in a high percentage of women. The HPV-11 cRIA titer appears to be a surrogate marker for neutralization
ISSN:0022-1899
1537-6613
DOI:10.1086/323645