QUANTIFICATION OF ELASTIN FROM THE MOUSE LUNG DURING POSTNATAL DEVELOPMENT

Elastic fibers play a vital role in secondary septal development during the process of lung alveolization. The authors present a quantitative study by image analysis and by high-performance liquid chromatography (HPLC) of the mouse lungs'elastic fibers throughout postnatal development. Five mic...

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Bibliographic Details
Published in:Experimental lung research 2001, Vol.27 (7), p.533-545
Main Author: Goncalves, Jose Barros, Ana Honorio, Paula Rodrigues, Vasco Bairos, Carlos
Format: Article
Language:English
Subjects:
Air breathing
Animals
Animals, Newborn
Biological and medical sciences
Chromatography, High Pressure Liquid
Elastic Fibers Elastin Desmosin Lung Development
Elastin - analysis
Fundamental and applied biological sciences. Psychology
Image Processing, Computer-Assisted - methods
Linear Models
Lung - chemistry
Lung - growth & development
Mice
Mice, Inbred Strains
Models, Animal
Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics
Vertebrates: respiratory system
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Summary:Elastic fibers play a vital role in secondary septal development during the process of lung alveolization. The authors present a quantitative study by image analysis and by high-performance liquid chromatography (HPLC) of the mouse lungs'elastic fibers throughout postnatal development. Five mice from birth up to the 21st day of postnatal development and on the 30th day of life were used daily. Their left lungs were collected and processed for observation under light microscopy. The staining was carried by a modification of the Gomori technique. The right lung of each animal was processed for elastin quantification by HPLC. Analysis by linear regression of the two methods' results showed there was a significant positive linear correlation. With this methodology of staining and image analysis we evaluated the normal establishment of the elastic network of the respiratory portion of the lung from birth to adult life. We now have a good model for studying the variations induced by exogenous agents in the synthesis and degradation of the pulmonary elastic fibers.
ISSN:0190-2148
1521-0499
DOI:10.1080/019021401753181818